Hiroyuki Shiojiri

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The effect of imidazole antimycotics (clotrimazole, econazole, and miconazole) on mitochondrial functions were compared by the experiment with isolated rat liver mitochondria and it was found that their potencies in the uncoupling effect on oxidative phosphorylation, in the stimulation of latent ATPase activity, and in the induction of swelling in(More)
The duclauxin derivatives xenoclauxin and desacetylduclauxin were examined for their effects on the growth of L-1210 murine leukemia cells, on the induction of DNA repair in the rat and mouse hepatocyte primary culture (HPC/DNA repair test), and on oxidative phosphorylation in mitochondria from rat livers in comparison to duclauxin. Both derivatives(More)
The delta 9-desaturase system in liver microsome from rats treated chronically with ethanol was studied. Stearoyl-CoA desaturase activity decreased by 80% and palmitoyl-CoA desaturase activity was not detectable in microsomes from ethanol-fed rats, while activities of electron transport components such as NADH-cytochrome c and NADH-ferricyanide reductases(More)
It was found that indomethacin, a potent inhibitor to cyclo-oxygenase in prostaglandin biosynthesis system, disturbed mitochondrial respiration and its farnesyl ester, E-0710 which no longer exhibited the inhibitory effect on cyclo-oxygenase, did not affect the mitochondrial respiration, indicating a good correlation between the inhibitory effect on ATP(More)
A high-performance liquid chromatographic method has been developed for the measurement of cholesterol 7 alpha-hydroxylase activity in liver microsomes. 7 alpha-Hydroxycholesterol generated from endogenous cholesterol was derivatized with anthroyl 1-carbonitrile, chromatographed on a reverse-phase column, and detected fluorometrically. The detection limit(More)
The interaction between BW755C (3-amino-1-[m-(trifluoromethyl)phenyl]-2-pyrazoline), a potent inhibitor of both lipoxygenase and cyclo-oxygenase, and respiratory chain in mitochondria and electron transport particles (ETP) from rat livers was examined. BW755C accelerated the oxygen uptake by mitochondria without the addition of substrate for the respiratory(More)
An antifungal antibiotic, pyrrolnitrin, was found to show very similar biological activities to those of imidazole antimycotics on isolated mitochondria. Pyrrolnitrin caused drastic swelling of mitochondria in isotonic solutions of alkali metal salts and of sucrose without any addition of substrate for the respiratory chain in mitochondria. The swelling(More)
The red blood cells from rats with intra- and extrahepatic cholestasis obtained by feeding of alpha-naphthylisothiocyanate (ANIT) and ligation of bile duct, respectively, were analysed from biochemical as well as ultrastructural point of view. A significant increase of cholesterol content was observed in the red cell membrane from bile duct-ligated rat. An(More)
The uncoupling activities of clidanac and its dechlorinated derivative on oxidative phosphorylation were examined using isolated rat liver mitochondria. Clidanac was found to uncouple the oxidative phosphorylation and no difference was detected in the uncoupling potency of the two enantiomorphs. Dechlorclidanac was significantly less effective in the(More)