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Germline stem cells that produce oocytes in vitro and fertilization-competent eggs in vivo have been identified in and isolated from adult mouse ovaries. Here we describe and validate a fluorescence-activated cell sorting-based protocol that can be used with adult mouse ovaries and human ovarian cortical tissue to purify rare mitotically active cells that(More)
A number of grammatical formalisms were introduced to define the syntax of natural languages. Among them are parallel multiple context-free grammars (pmcfg's) and lexical-functional grammars (lfg's). Pmcfg's and their subclass called multiple context-free grammars (mcfg's) are natural extensions of cfg's, and pmcfg's are known to be recognizable in(More)
Several grammars have been proposed for modeling RNA pseudoknotted structure. In this paper, we focus on multiple context-free grammars (MCFGs), which are natural extension of context-free grammars and can represent pseudoknots, and extend a specific subclass of MCFGs to a probabilistic model called SMCFG. We present a polynomial time parsing algorithm for(More)
Several grammars have been proposed for modeling RNA pseudoknotted structure. In this paper, we focus on multiple context-free grammars (MCFGs), which are natural extension of context-free grammars and can represent pseudoknots, and extend a specific subclass of MCFGs to a probabilistic model called SMCFG. We present a polynomial time parsing algorithm for(More)
PURPOSE High-grade serous ovarian cancers are heterogeneous not only in terms of clinical outcome but also at the molecular level. Our aim was to establish a novel risk classification system based on a gene expression signature for predicting overall survival, leading to suggesting novel therapeutic strategies for high-risk patients. EXPERIMENTAL DESIGN(More)