Hirotoshi Seguchi

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Electron microscopic and morphometric investigations on undescended testicles show a decreased volume density of the spermatogonia up to the age of 5 (approximately 60%, p smaller than 0.005), corresponding to investigations by light microscopy. There are no ultrastructural differences in the seminiferous tubules between normal and undescended testicles up(More)
BACKGROUND Reports concerning the immunological functions of lymphocytes derived from umbilical cord blood cell (UCBC) have been limited. METHODS In murine syngeneic transplantation system using green fluorescent protein transgenic donors, UCBC-derived lymphocytes were studied for their immunological competence. RESULTS Hematopoietic stem cells (HSC)(More)
1. By use of the reverse transcription polymerase chain reaction (RT-PCR), we determined the expression of adrenomedullin (AM) mRNA in the various tissues of the pig. To evaluate the significance of the expression of AM mRNA, we also determined the effects of AM on the cytosolic Ca2+ concentration ([Ca2+]i) and tension development of the porcine smooth(More)
PURPOSE To investigate the expression of beta-adrenoceptor (AR) subtypes responsible for detrusor smooth muscle relaxation. MATERIALS AND METHODS Isolated rat detrusor smooth muscle was examined by tension measurement and reverse transcription polymerase chain reaction (RT-PCR). RESULTS Norepinephrine (NE), epinephrine (EP) and isoproterenol (ISO) were(More)
We tested the hypothesis that adrenomedullin (ADM), a recently isolated hypotensive peptide, is an autocrine transmitter in renal arterial smooth muscle cells (RASMCs). Fura-2 fluorometry revealed that ADM decreases both the cytosolic Ca2+ concentration ([Ca2+]i) and tension induced by 0.3 microM phenylephrine (PE) in porcine RASMCs. The expression of ADM(More)
We investigated the expression and function of alpha1-adrenoceptor subtypes in the porcine renal artery. Reverse transcription polymerase chain reaction (RT-PCR) and nucleotide sequencing indicated that the mRNAs for alpha1a- and alpha1b-adrenoceptors were expressed in the porcine renal artery. Chloroethylclonidine, an alpha1B- and alpha1D-adrenoceptor(More)