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Isolation and characterization of styrene metabolism genes from styrene-assimilating soil bacteria Rhodococcus sp. ST-5 and ST-10.

• Hiroshi Toda, Nobuya Itoh
• Journal of bioscience and bioengineering
• 2012

Styrene metabolism genes were isolated from styrene-assimilating bacteria Rhodococcus sp. ST-5 and ST-10. Strain ST-5 had a gene cluster containing four open reading frames which encoded styrene… (More)

Expression of Clostridium acetobutylicum butanol synthetic genes in Escherichia coli

• Masayuki Inui, Masako Suda, +7 authors Hideaki Yukawa
• Applied Microbiology and Biotechnology
• 2007

A recombinant butanol pathway composed of Clostridium acetobutylicum ATCC 824 genes, thiL, hbd, crt, bcd-etfB-etfA, and adhe1 (or adhe) coding for acetyl-CoA acetyltransferase (THL),… (More)

Overexpression of genes encoding glycolytic enzymes in Corynebacterium glutamicum enhances glucose metabolism and alanine production under oxygen deprivation conditions.

• Shogo Yamamoto, Wataru Gunji, +5 authors Hideaki Yukawa
• Applied and environmental microbiology
• 2012

We previously reported that Corynebacterium glutamicum strain ΔldhAΔppc+alaD+gapA, overexpressing glyceraldehyde-3-phosphate dehydrogenase-encoding gapA, shows significantly improved glucose… (More)

Involvement of S-adenosylmethionine-dependent halide/thiol methyltransferase (HTMT) in methyl halide emissions from agricultural plants: isolation and characterization of an HTMT-coding gene from…

• Nobuya Itoh, Hiroshi Toda, Michiko Matsuda, Takashi Negishi, Tomokazu Taniguchi, Noboru Ohsawa
• BMC Plant Biology
• 2009

Biogenic emissions of methyl halides (CH3Cl, CH3Br and CH3I) are the major source of these compounds in the atmosphere; however, there are few reports about the halide profiles and strengths of these… (More)

Expression and characterization of styrene monooxygenases of Rhodococcus sp. ST-5 and ST-10 for synthesizing enantiopure (S)-epoxides

• Hiroshi Toda, Ryouta Imae, Tomoko Komio, Nobuya Itoh
• Applied Microbiology and Biotechnology
• 2011

Styrene monooxygenase (StyA, SMOA)- and flavin oxidoreductase (StyB, SMOB)-coding genes of styrene-assimilating bacteria Rhodococcus sp. ST-5 and ST-10 were successfully expressed in Escherichia… (More)

Microbial production of aliphatic (S)-epoxyalkanes by using Rhodococcus sp. strain ST-10 styrene monooxygenase expressed in organic-solvent-tolerant Kocuria rhizophila DC2201.

• Hiroshi Toda, Takuya Ohuchi, Ryouta Imae, Nobuya Itoh
• Applied and environmental microbiology
• 2015

We describe the development of biocatalysis for producing optically pure straight-chain (S)-epoxyalkanes using styrene monooxygenase of Rhodococcus sp. strain ST-10 (RhSMO). RhSMO was expressed in… (More)

Identification and characterization of a novel (−)-vibo-quercitol 1-dehydrogenase from Burkholderia terrae suitable for production of (−)-vibo-quercitol from 2-deoxy-scyllo-inosose

• Nobuya Itoh, Junji Kurokawa, Hiroshi Toda, Kazunobu Konishi
• Applied Microbiology and Biotechnology
• 2017

(−)-vibo-Quercitol is a deoxyinositol (1l-1,2,4/3,5-cyclohexanepentol) that naturally occurs in oak species, honeydew honey, wines aged in oak barrels, and Gymnema sylvestre and is a potential… (More)

Molecular cloning and characterization of a flavonoid-O-methyltransferase with broad substrate specificity and regioselectivity from Citrus depressa

• Nobuya Itoh, Chisa Iwata, Hiroshi Toda
• BMC Plant Biology
• 2016

Flavonoids are secondary metabolites that play significant roles in plant cells. In particular, polymethoxy flavonoids (PMFs), including nobiletin, have been reported to exhibit various… (More)

Isolation and characterization of a gene encoding a S-adenosyl-l-methionine-dependent halide/thiol methyltransferase (HTMT) from the marine diatom Phaeodactylum tricornutum: Biogenic mechanism of…

• Hiroshi Toda, Nobuya Itoh
• Phytochemistry
• 2011

Several marine algae including diatoms exhibit S-adenosyl-l-methionine (SAM) halide/thiol methyltransferase (HTMT) activity, which is involved in the emission of methyl halides. In this study, the in… (More)

Characterization of two cryptic plasmids from Kocuria palustris IPUFS-1 and construction of novel Escherichia coli-Kocuria shuttle vector for biocatalysis.

• Hiroshi Toda, Takashi Koyanagi, Toshiki Enomoto, Nobuya Itoh
• Journal of bioscience and bioengineering
• 2017

Two cryptic plasmids, designated pKPAL1 and pKPAL2, were identified from the gram-positive bacterium Kocuria palustris IPUFS-1, which was isolated from a fish source. The 2251-bp and 2488-bp circular… (More)

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