Hiroshi Sekiguchi

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Chitin samples in a alpha-form structure were isolated from beetle larva cuticle and silkworm (Bombyx mori) pupa exuvia by treatment with 1 N HCl and 1 N NaOH. Chitosan was prepared by treating them in 40% NaOH containing NaBH(4). Chitin and chitosan were analyzed by X-ray, [13C]CP/MAS NMR, [13C]FT-NMR, and scanning electron microscopy (SEM) methods. Insect(More)
An obligately aerobic, Gram-negative, non-spore-forming, non-motile, spherical bacterium, designated strain 04OKA010-24(T), was isolated from seawater surrounding the hard coral Galaxea fascicularis L., collected at Majanohama, Akajima, Japan, and was subjected to a polyphasic taxonomic study. Phylogenetic analyses based on the 16S rRNA gene sequence(More)
A taxonomic study was carried out to clarify the status of a Gram-negative, heterotrophic mesophile that was isolated from the marine sponge Halichondria okadai. The strain, designated HOact23(T), was a non-motile, rod-shaped (0.44-0.53x0.65-0.79 microm) bacterium. The strain produced squalene and a red-pink carotenoid pigment. The cell-wall peptidoglycan(More)
Classical swine fever virus (CSFV) strain WB82, isolated from a wild boar in 1982, induced a distinct cytopathic effect (CPE) in primary swine testicle cell culture and in most of the porcine cell lines. This strain of CSFV was found to be composed of two biotypes. cytopathogenic (cp) CSFV, as a minor population, and noncytopathogenic (noncp) CSFV, as a(More)
INTRODUCTION Quality of information available over the Internet has been a cause for concern. Our goal was to evaluate the quality of information available on lung cancer in the United States and Japan and assess the differences between the two. METHODS We conducted a prospective, observational Web review by searching the word "lung cancer" in Japanese(More)
The dynamics of a single protein molecule subjected to forced mechanical unfolding was investigated in a millisecond time domain using a custom-made atomic force microscope (AFM) apparatus, which allows simultaneous measurements of an average tensile force applied to a single molecule and its mechanical response with respect to an external oscillation. Our(More)
To establish a practical method for detecting porcine epidemic diarrhea virus (PEDV), the use of primers derived from sequences that amplify the M protein genes of PEDV in a RT-PCR detection system was investigated. Primers were designed to amplify a 854-bp fragment by RT-PCR. This reaction was specific to the PEDV RNA but not to that of other viral genera(More)
The cellular response to external mechanical forces has important effects on numerous biological phenomena. The sequences of molecular events that underlie the observed changes in cellular properties have yet to be elucidated in detail. Here we have detected the responses of a cultured cell against locally applied cyclic stretching and compressive forces,(More)
In a series of experiments, we have tested the usefulness and limitations of the Guardian Bio-Threat Alert (BTA) system for detection of staphylococcal enterotoxin B (SEB), botulinum toxins (BTXs) A and B, and ricin. In this report, the BTA system has been further evaluated for toxin subtypes and the detection ability of manufactural lots of the BTA strips.(More)