Hiroshi Miyazaki

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Dehydroepiandrosterone (DHEA) is a naturally occurring steroid synthesized in the adrenal cortex, gonads, brain, and gastrointestinal tract, and it is known to have chemopreventive and anti-proliferative actions on tumors. These effects are considered to be induced by the inhibition of glucose-6-phosphate dehydrogenase (G6PD) and/or HMG-CoA reductase (HMGR)(More)
We have developed a new sensitive and specific nonradioisotope assay method to measure the activity of HMG-CoA reductase, the rate-controlling enzyme in the cholesterol biosynthetic pathway. This method was based upon a stable isotope dilution technique by liquid chromatography-tandem mass spectrometry using electrospray ionization in positive mode.(More)
We developed a method to reconstruct cell geometry from confocal fluorescence microscopy images of the cytoskeleton. In the method, region growing was implemented twice. First, it was applied to the extracellular regions to differentiate them from intracellular noncytoskeletal regions, which both appear black in fluorescence microscopy imagery, and then to(More)
The advance of UML® 2.0 standardization work by OMG™ provides a good opportunity for ODP community to leverage UML 2.0 to show the value of ODP. In this paper, we examine issues in applying UML 2.0 to ODP Engineering and Technology Viewpoint Languages, and show how we may be able to use UML to represent those ODP Viewpoint specifications.
The ODP Enterprise Language is used to describe the organizational objectives and policies that apply to the system to be specified. It also captures constraints associated with the environment in which the system is to be used. Because the enterprise specification is concerned more with organizational issues than technical details of the system, there is(More)
We demonstrate an active camera system for high-speed object tracking using a high-speed camera that can take an image sequence at a rate of 1000 frame/s. By making effective use of properties of high-frame-rate image sequences, our tracking algorithm enables us to robustly track a fast moving object in a typical indoor environment that contains a cluttered(More)
ii Acknowledgements First and foremost, I would like to thank my primary investigator, friend, and mentor, Dr. Hiroshi Miyazaki. His vast knowledge and dedication to cancer biology is bar none. Between his analogies, explanations, assistance, and unending patience, his persistence and robust methodologies in the laboratory stood out the most. I would also(More)
We developed a mechanical tissue model containing a cell with cytoskeletal filaments inside to investigate how tissue deformation is reflected in the deformation of a cell and its internal cytoskeletal filaments. Tissue that assumes a collagen gel construct was depicted as an isotropic linear elastic material, and the cell was modeled as an assembly of(More)