Hirohisa Shiojiri

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The effect of imidazole antimycotics (clotrimazole, econazole, and miconazole) on mitochondrial functions were compared by the experiment with isolated rat liver mitochondria and it was found that their potencies in the uncoupling effect on oxidative phosphorylation, in the stimulation of latent ATPase activity, and in the induction of swelling in(More)
An antifungal antibiotic, pyrrolnitrin, was found to show very similar biological activities to those of imidazole antimycotics on isolated mitochondria. Pyrrolnitrin caused drastic swelling of mitochondria in isotonic solutions of alkali metal salts and of sucrose without any addition of substrate for the respiratory chain in mitochondria. The swelling(More)
It was found that indomethacin, a potent inhibitor to cyclo-oxygenase in prostaglandin biosynthesis system, disturbed mitochondrial respiration and its farnesyl ester, E-0710 which no longer exhibited the inhibitory effect on cyclo-oxygenase, did not affect the mitochondrial respiration, indicating a good correlation between the inhibitory effect on ATP(More)
Automatic generation of textured object models from a sequence of range and color images requires two major tasks: measurement registration and measurement integration. Measurement registration is the estimation of the current position and orientation of the object in 3D space with respect to an arbitrary fixed reference, given the current measurement and(More)
A high-performance liquid chromatographic method has been developed for the measurement of cholesterol 7 alpha-hydroxylase activity in liver microsomes. 7 alpha-Hydroxycholesterol generated from endogenous cholesterol was derivatized with anthroyl 1-carbonitrile, chromatographed on a reverse-phase column, and detected fluorometrically. The detection limit(More)
The delta 9-desaturase system in liver microsome from rats treated chronically with ethanol was studied. Stearoyl-CoA desaturase activity decreased by 80% and palmitoyl-CoA desaturase activity was not detectable in microsomes from ethanol-fed rats, while activities of electron transport components such as NADH-cytochrome c and NADH-ferricyanide reductases(More)
The surface temperature of a rat's paw is greatly affected by heat transfer because of its contacts with the body, as well as several environmental conditions. To establish a methodology for determining the surface temperature of the inflamed rat's paw, we devised an individual cage with a pair of rolling rods. The rods were set to protect contacts of paws(More)
The red blood cells from rats with intra- and extrahepatic cholestasis obtained by feeding of alpha-naphthylisothiocyanate (ANIT) and ligation of bile duct, respectively, were analysed from biochemical as well as ultrastructural point of view. A significant increase of cholesterol content was observed in the red cell membrane from bile duct-ligated rat. An(More)
The effect of a novel antiatheromatous agent, N-(3-[4'-(2'',6''-dimethylheptyl)phenyl]butanoyl)ethanolamin e (E5050), on the proliferation of porcine aortic smooth muscle cells was studied in vitro. E5050 dose-dependently inhibited DNA synthesis as well as proliferation of cells stimulated with 10% fetal calf serum with no cytotoxic effects. An inhibitory(More)