Hiroe Miyamoto

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The effect of direct electrical stimulation on suspensions of sarcoplasmic reticulum membrane fragments (SRF) was carefully re-examined using the method of Lee et al. (1966) J. Gen. Physiol. 49:689. Inhibition of Ca(++) uptake or release by electrical stimulation was observed. When platinum electrodes were used as stimulating electrodes, the effect was(More)
In order to prevent postoperative adhesion and the related complications, a thermally crosslinked gelatin (TCG) film was developed and the basic biological properties were examined, paying special attention to the relationship between these properties and the extent of crosslinking of the film. The gelatin films crosslinked thermally for five different time(More)
  • H Miyamoto
  • 1986
This paper reports the survival of mouse embryos after freezing when glycol derivatives or erythritol is used as the cryoprotectant and also describes the two-step preservation of mouse embryos (rapid cooling to solid CO2 or liquid nitrogen vapor before transfer to liquid nitrogen). Ethylene glycol was the most effective cryoprotectant of glycols tested and(More)
AIM Adhesion after pelvic surgery causes infertility, ectopic pregnancy, and ileus or abdominal pain. The materials currently available for clinical use are insufficient. The purpose of this study was to develop an anti-adhesive material that overcomes the limitations of conventional anti-adhesive agents. METHODS The adhesion prevention effects of three(More)
To create more useful, effective and safer anti-adhesion materials, we developed a thermally cross-linked gelatin film. In this study, we examined the physical properties of the film such as the physical strength and the adhesiveness to reveal the handling properties and biological properties, such as the anti-adhesion effect, the influence on cell(More)
Postoperative intra-abdominal or intrathoracic adhesions sometimes cause significant morbidity. We have designed three types of alginate-based treatments using strongly cross-linked (SL), weakly cross-linked (WL), and non-cross-linked (NL) alginate with calcium gluconate. In rat experiments, we compared the antiadhesive effects of the three types of(More)
An image analysis system, assigned different pseudocolors to different types of immunolabeled cells, allowed us to make a montage from two images of the respective types of cells. This system was therefore used for simultaneous identification of two or more types of immunolabeled cells in the sheep anterior pituitary. Morphometry--including a neighboring(More)
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