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DNA methylation is globally reprogrammed during mammalian preimplantation development, which is critical for normal development. Recent reduced representation bisulfite sequencing (RRBS) studies suggest that the methylome dynamics are essentially conserved between human and mouse early embryos. RRBS is known to cover 5-10% of all genomic CpGs, favoring(More)
DNA fragments of 8.5 kb containing the gyrA gene were cloned from Escherichia coli KL-16 and from four spontaneous gyrA mutants which showed various levels of resistance to quinolones. The gyrA gene was situated at about 4 kb in front of the nrdA gene and transcribed counterclockwise on the E. coli chromosome. It encoded a polypeptide of 875 amino acids(More)
OBJECTIVE To evaluate viability and subsequent developmental ability in mouse germinal vesicle (GV) oocytes ultrarapidly vitrified with serial stepwise exposure. DESIGN Experimental animal study. SETTING University-based research laboratory. ANIMAL(S) Three- to 4-week-old female (C57BL/6J x CBA) F1 mice in a laboratory environment. INTERVENTION(S)(More)
The purpose of this study was to evaluate the viability and subsequent developmental ability of murine germinal vesicle (GV) oocytes ultrarapidly vitrified after step-wise exposure to cryoprotectants (CPAs). Oocytes were transferred to a vitrification solution composed of 15% ethylene glycol, 15% dimethyl sulfoxide and 0.5 M sucrose in a direct manner(More)
Low-dose macrolides are effective therapy in patients with chronic lung infections, but the mechanisms of action are unclear. In this study, we performed DNA microarray analysis of Pseudomonas aeruginosa after treatment with a low concentration of azithromycin. We found that a sub-MIC of azithromycin didn't change mRNA expression of quorum-sensing related(More)
The three-dimensional (3D)-engineered tissues composed of only cells and extracellular matrices (ECM) were constructed by the hydrogel template approach. The disulfide-crosslinked poly(gamma-glutamic acid) hydrogels were prepared as a template hydrogel. These template hydrogels were easily decomposed under physiological conditions using reductants such as(More)
Search-based program repair automatically searches for a program fix within a given repair space. This may be accomplished by retrofitting a generic search algorithm for program repair as evidenced by the GenProg tool, or by building a customized search algorithm for program repair as in SPR. Unfortunately, automated program repair approaches may produce(More)
DNA fragments of 3.4 kb containing the gyrB gene were cloned from Escherichia coli KL-16 and from spontaneous nalidixic acid-resistant mutants. The mutations (nal-24 and nal-31) had been determined to be in the gyrB gene by transduction analysis. Nucleotide sequence analysis of the cloned DNA fragments revealed that nal-24 was a G to A transition at the(More)
Since the tactile perception detects skin deformation due to the contact of an object, it is important to understand contact mechanics, especially, frictional behavior of the human fingertip. The coefficient of friction is recently modeled as a function of the applied normal load in which case the traditional Coulomb's law does not provide a description for(More)