Himangshu Raj

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OBJECTIVES The present study was carried out to investigate the in vivo antimycobacterial activity of methanol extract of Alstonia scholaris and Mucuna imbricata in murine model. MATERIALS AND METHODS Female BALB/c mice were infected with the Mycobacterium tuberculosis H37Rv suspension. Extracts were administered orally for 2 weeks from 7th day(More)
The objective of this study was to examine expression of certain developmental genes in bovine 2cell stage embryos. Bovine oocytes were recovered from abattoir originated ovaries and subjected for maturation and fertilization in vitro. mRNA extraction and subsequent reverse transcriptase PCR was done to evaluate expression of GJA1, ACTB, PAPOLA and POU5F1(More)
Aim: To evaluate the developmental competence of post-thaw vitrified bovine cumulus-oocyte complexes (COCs) in vitro. Materials and Methods: A total of 129 COCs were cryopreserved using vitrification solution comprising of 15% ethylene glycol (EG) + 15% dimethyl sulfoxide (DMSO) + 0.6 M sucrose in medium TCM-199 with 10% FBS. Immediately, within a minute(More)
AIM Thecurrent study aims at investigating the polyadenylated (poly[A]) tail length of morphologically high and low competent oocytes at different developmental stages. Furthermore, effect of ultra-rapid vitrification on the poly(A) tail length was studied. MATERIALS AND METHODS Fresh bovine cumulus oocyte complexes from abattoir originated ovaries were(More)
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