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An immunoassay for the determination of human placental lactogen (HPL) has been developed using specific antibodies labelled with horseradish peroxidase. A method is presented in which the enzyme : antibody conjugate is purified with an antigenic solid phase to ensure the production of conjugates with intact immunological activity. Serum components(More)
Because of the difficulties associated with obtaining patient samples and the labile nature of some analytes, manufacturers will always require the assistance of clinical chemistry laboratories in the establishment of performance claims, but our experience suggests that this work should not be undertaken lightly by laboratories and that manufacturers would(More)
A method for the simultaneous determination of sweat sodium and chloride was developed and evaluated using a Monarch 2000 centrifugal analyser with ion-selective electrodes. In order to measure sodium and chloride in diluted sweat samples and sweat paper eluates an analytical working range of below 10 mmol/L was required. This was achieved by modification(More)
A kinetic assay for urine urea which employs the reaction with diacetylmonoxime at 37 degrees C and undiluted urine was developed. Reaction conditions were optimized using the Monarch centrifugal analyser to produce an assay with the linearity to 450 mmol/L urea, and CVs of 2.3% and 2.5% within-batch and 4.6% and 3.3% between-batch at urea concentrations of(More)
The "Magnesium Liquid Stable Reagent Set" from Medical Analysis Systems, Inc., is evaluated. The method, which involves Magon dye binding and bichromatic absorbance measurements, was used in a Monarch centrifugal analyzer. Results were compared with those by atomic absorption spectrometry. The calibration curve for the Magon method is linear to 2.5 mmol/L,(More)
We investigated the measurement of phenytoin in plasma by the EMIT method, in the Monarch centrifugal analyzer. When we used the standard protocol supplied by Instrumentation Laboratory, significant drift was observed across a full rotor loaded with 31 replicates of a single specimen. Although the cause remains obscure, the drift was eliminated by using a(More)
This paper describes the field performance of the Kodak EKTACHEM GLU/BUN Analyzer for glucose and urea. NCCLS protocols PSEP-2, 3 an 4 were used which enable manufacturers to establish performance claims concerning the precision and accuracy of an analytical system. This multicentre trial used four analysers in four European countries, United Kingdom,(More)