Hilary A Macqueen

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Rats were fed from weaning on chow supplemented with suet or sunflower oil (10 % (w/w) each). The appearance of receptors for tumour necrosis factor-alpha on perinodal adipocytes from the popliteal depot following a subcutaneous injection of bacterial lipopolysaccharide was examined. In rats fed on sunflower oil-supplemented chow receptors appeared at a(More)
Synthesis of laminin by mouse preimplantation embryos was examined by specific immunoprecipitation of [35S]methionine-labeled cell lysates. The three polypeptide subunits of laminin, A, B1, and B2, are not necessarily synchronously expressed during development. Oocytes and eggs synthesize only one immunoprecipitable polypeptide, which comigrates with the(More)
We used immunohistochemical techniques to demonstrate the distribution of receptors for the cytokine tumour necrosis factor-alpha on the popliteal lymph node and the adipose tissue surrounding it for 5 d following a simulated immune challenge to one hind leg in the rat. We found different patterns of expression of receptors on adipocytes surrounding a lymph(More)
Preimplantation mouse embryos were metabolically labelled with 3H or 14C-fucose to investigate the synthesis of fucosylated macromolecules. Scintillation counting revealed that there was a progressive increase in both total fucose taken up by the embryo and incorporation of fucose into TCA-precipitable material as embryos developed from the 4-cell to the(More)
We report a change in the vascularisation of the adipose depots surrounding the popliteal lymph node that has, and the contralateral node that has not, been exposed to a simulated immune challenge. The percentage of the depot that consists of vessels, as measured by image analysis, decreases over a period of 2 d after immune stimulus, then increases in a(More)
Adipogenesis is a complex process that involves the differentiation of preadipocytes into mature adipocytes. We have developed two-dimensional (2D) and three-dimensional (3D) cell culture systems for the purpose of culturing and differentiating primary preadipocytes in vitro. Differentiating preadipocytes show multiple lipid droplet accumulation and(More)
Newly-formed pairs of 16-cell blastomeres were collected by periodic observation of isolated 8-cell blastomeres. Any pairs formed by division were recovered and classified as being composed of 1/16 blastomeres that differed in size or were of similar size. All of the latter and some of the former were then cultured for periods of up to 20h. The remaining(More)
The development of pre-implantation mouse embryos was found to be prevented by exposure of the embryos to [35S]methionine, but not to [3H]methionine. Such embryos have also been shown to be highly sensitive to [3H]thymidine. These observations are discussed with reference to the path lengths and energies of electrons emitted from the different radioisotopes.
Single-photon counting fluorimetry was used to record the time course of the expression of interleukin-10 receptors labelled with fluorescent antibodies on the surface of adipocytes over 24h, following an immune challenge to the rat popliteal lymph node. Homologous perinodal and remote-from-node samples from the stimulated and unstimulated popliteal depots(More)