Hidetaro Yasumitsu

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Proli'¡misespecies secreted by 10 human gastric carcinoma cell lines were analyzed by gelatin zymography and immunoblotting. These cell lines were classified into the following three groups with respect to proteinase secretion: cell lines secreting mainly gelatinases A and/or B; those secreting multiple types of serine proteinases; and those scarcely(More)
A metalloproteinase with M, 29,000 was purified to homogeneity as a latent proenzyme from the conditioned medium of a human rectal carci noma cell line CaR-1. This enzyme hydrolyzed casein more potently than gelatin embedded in polyacrylamide gels in zymography assay. Calcium ion was essential for the activity. It exerted the maximum activity at pH 7-9. Its(More)
SBL/RC-RNase was originally isolated from frog (Rana catesbeiana) oocytes and purified as a novel sialic acid-binding lectin (SBL) that displayed strong anti-cancer activity. SBL was later shown to be identical to a ribonuclease (RC-RNase) from oocytes of the same species. The administration of SBL/RC-RNase induced apoptosis (with nuclear condensation and(More)
A divalent cation-independent lectin-HOL-18, with cytotoxic activity against leukemia cells, was purified from a demosponge, Halichondria okadai. HOL-18 is a 72 kDa tetrameric lectin that consists of four non-covalently bonded 18 kDa subunits. Hemagglutination activity of the lectin was strongly inhibited by chitotriose (GlcNAcβ1-4GlcNAcβ1-4GlcNAc), fetuin(More)
A D-galactose specific lectin purified from the eggs of sea hare, Aplysia kurodai (AKL) by lactosyl-agarose affinity chromatography has been evaluated for screening of antimicrobial activities. AKL was disulfide bonded dimeric lectin consisted of two 32 kDa polypeptides. This lectin has significant hemagglutinting activity against trypsinized rabbit and(More)
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