Learn More
Antifungal activity of the novel compound acibenzolar-S-methyl (CGA245704: benzo[1,2,3]thiadiazole-7-carbothioic acid S-methyl ester) was examined in vitro. No remarkable activity was observed on mycelial growth and conidial germination of almost all fungi tested. Only melon isolates of Didymella bryoniae were sensitive to this compound. On potted plants,(More)
ABSTRACT The infection of Japanese pear by Venturia nashicola, the cause of scab on Asian pears (Japanese pear, Pyrus pylifolia var. culta; Chinese pear, P. ussuriensis), was examined using light and electron microscopy to determine the mechanism of resistance in pears. Early stages of infection were similar on the susceptible cv. Kosui, the resistant cv.(More)
BACKGROUND QoI fungicides, inhibitors of mitochondrial respiration, are considered to be at high risk of resistance development. In several phytopathogenic fungi, resistance is caused by mutations (most frequently G143A) in the mitochondrial cytochrome b (cytb) gene. The genetic and molecular basis of QoI resistance were investigated in laboratory and field(More)
Wheat head blight pathogens Fusarium graminearum and Microdochium nivale have distinct sensitivities to strobilurin fungicides, which inhibit activity of complex III in the mitochondrial electron transport chain. When mycelia were cultured in medium with the strobilurin fungicide azoxystrobin (AZ), F. graminearum increased its oxygen-consumption, but M.(More)
Sample preparation in plant proteomics is tedious, requiring modifications depending on the type of tissue involved. Here, we describe a protein extraction protocol for both monocotyledonous (monocot) and dicotyledonous (dicot) species, which significantly improves the solubilization of total proteins. For example, we used the primary leaf tissue and seeds(More)
BACKGROUND In 2004, field isolates of Botrytis cinerea Pers. ex Fr., resistant to strobilurin fungicides (QoIs), were first found in commercial citrus orchards in Wakayama Prefecture, Japan. Subsequently, QoI-resistant isolates of this fungus were also detected in plastic strawberry greenhouses in Saga, Ibaraki and Chiba prefectures, Japan. Biological and(More)
ABSTRACT This study reports the mode of action of acibenzolar-S-methyl (ASM) against Japanese pear scab, caused by Venturia nashicola. Pretreatment of potted Japanese pear trees with ASM reduced scab symptoms and potentiated several lines of plant defense response. This included transcripts encoding polygalacturonase-inhibiting protein (PGIP) that were(More)
This article is an abstract of the paper presented by a winner of the Society Fellowship at the 2004 Annual Meeting of the Phytopathological Society of Japan in Fukuoka In 1971 polyoxin-resistant isolates of Alternaria alternata Japanese pear pathotype and isolates of Magnaporthe grisea, resistant to kasugamycin, were found in Tottori Prefecture and(More)
A gene encoding a leucine-rich repeat receptor-like protein kinase (LRPK) was identified from Japanese pear (Pyrus pyrifolia var. culta) using reverse transcription-polymerase chain reaction (RT-PCR) and rapid amplification of cDNA ends (RACE) PCR. The Japanese pear LRPKp protein was composed of a leucine-rich extracellular ligand-binding domain, a(More)
Fungicide resistance in plant pathogens is often caused by a single point mutation in a gene encoding fungicide target proteins. Such is the case for resistance to MBI-D (inhibitors of scytalone dehydratase in melanin biosynthesis) fungicides in rice blast fungus (Magnaporthe oryzae), which is caused by a mutation in the scytalone dehydratase gene that(More)