Hidenari Yamada

Learn More
Sustained smooth muscle contraction is mediated by protein kinase C (PKC) through a signal transduction cascade leading to contraction. Heat-shock protein 27 (HSP27) appears to be the link between these two major events, i.e., signal transduction and sustained smooth muscle contraction. We have investigated the involvement of HSP27 in signal transduction(More)
The mammalian phospholipase A2-activating protein (PLAP) affects of smooth muscle cells isolated from the rabbit rectosigmoid. PLAP (10(6) M)-induced contraction peaked at 30 sec and was sustained at 4 min. MAP kinase was activated by PLAP (10(-6) M), as measured using myelin basic protein (MBP) as substrate. The increase in MAP kinase activity was rapid at(More)
In Schwann cells, the transmembrane glycoprotein β-dystroglycan composes the dystroglycan complex together with the extracellular glycoprotein α-dystroglycan, which binds laminin-2 (α2/β1/γ1), a major component of the Schwann cell basal lamina. In the Schwann cell cytoplasm, β-dystroglycan is anchored to a dystrophin isoform, Dp116. In this study, we(More)
We have investigated the hypotheses that 1) bombesin activation of protein kinase C (PKC) results in the hydrolysis of sphingolipids and the production of ceramide and that 2) ceramide produced on activation by bombesin mediates sustained contraction of smooth muscle cells by activation of PKC and mitogen-activated protein (MAP) kinase. Ceramide production(More)
We have investigated whether mitogen-activated protein (MAP) kinase cascade is essential for sustained contraction of smooth muscle cells of the rabbit rectosigmoid. We have identified MAP kinase as one of the enzymes activated by bombesin, performed immunologic studies blocking the activation of MAP kinase, and conducted confocal localization of MAP kinase(More)
We have investigated the effect of sphingosylphosphorylcholine (SPC), a synthetic product that was implicated in the sphingomyelin cycle, and have assessed its role in intracellular signaling. SPC induced a dose-dependent contractile effect of smooth muscle cells isolated from the rectosigmoid of the rabbit. Maximal contraction occurred at 10(-6) M. The(More)
Three foldases--the apical domain of GroEL (mini-chaperone) and two oxidoreductases (DsbA and DsbC) from Escherichia coli--were studied in refolding a protein with immunoglobulin fold (immunoglobulin-folded protein) that had been produced as inclusion bodies in E.coli. The foldases promoted the refolding of single-chain antibody fragments from(More)
  • 1