Hidekatsu Ito

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The authors studied effects of opioid receptor agonists on neuronal nicotinic-receptor-mediated current in PC12 cells using whole-cell current recording. At 1 microM, [d-Ala, N-Me, Phe, Gly-ol]- enkephalin (DAMGO), a selective micro receptor agonist, or 10 microM methionine-enkephalin, a micro and delta receptor agonist, did not inhibit the current elicited(More)
BACKGROUND Alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-type glutamate receptors mediate fast excitatory synaptic transmission in the central nervous system. Although barbiturates have been shown to suppress the AMPA receptor-mediated responses, it is unclear whether this effect contributes to the anesthetic action of barbiturates. The(More)
Dog myoblasts obtained from muscle biopsies were infected in vitro with a defective retroviral vector containing a cytoplasmic beta-galactosidase (beta-Gal) gene. These myoblasts were initially transplanted in the irradiated muscles of SCID mice and beta-Gal positive muscle fibers were observed. beta-Gal myoblasts were also transplanted back either in the(More)
To investigate relationships between neuronal network activity and electrical stimulus, we analyzed autonomous activity before and after electrical stimulus. Recordings of autonomous activity were performed using dissociated culture of rat hippocampal neurons on a multi-electrodes array (MEA) dish. Single stimulus and pared stimuli were applied to a(More)
Spatiotemporal pattern of neuronal network activity is a key component of brain information processing. Cultured rat hippocampal neurons on the multielectrodes array dish are suitable for analyzing and manipulating network dynamics and its developmental changes. We applied paired electrical inputs at various inter-stimulus intervals (ISi) and analyzed the(More)
beta-Adrenergic regulation of exocrine protein secretion from the parotid gland was studied over the adult rat life span. Enzymatically dispersed cell aggregates were prepared from 3-, 6-, 12-, and 24-month-old rats and exocrine protein secretion (amylase release) measured. No age differences were seen in the time course of amylase release following(More)
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