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Selenophosphate synthetase genes from lung adenocarcinoma cells: Sps1 for recycling L-selenocysteine and Sps2 for selenite assimilation.
A labile selenium donor compound monoselenophosphate is synthesized from selenide and ATP by selenophosphate synthetase (SPS). In the present study, Sps1 and Sps2 were cloned from a cDNA libraryExpand
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Purification of bacterial l-methionine γ-lyase
Abstract A rapid procedure for the purification of l -methionine γ-lyase from Pseudomonas putida ICR 3460 by DEAE-TOYOPEARL 650M and DEAE-Sephadex A-50 column chromatography is presented. The enzymeExpand
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Alanine Racemase from an Acidophile, Acidiphilium organovorum: Purification and Characterization.
An alanine racemase (EC 5.1.1.1) from an acidophilic heterotrophic bacterium, Acidiphilium organovorum 13H, was purified and characterized. The enzyme had a dimeric structure with identical subunitsExpand
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Plant-based Paste Fermented by Lactic Acid Bacteria and Yeast: Functional Analysis and Possibility of Application to Functional Foods
A plant-based paste fermented by lactic acid bacteria and yeast (fermented paste) was made from various plant materials. The paste was made of fermented food by applying traditional food-preservationExpand
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Chemical modification of cysteine residues of L-methionine γ-lyase
The L-methionine γ-lyase of Pseudomonas putida contains 16 cysteinyl residues per mol of enzyme, which is composed of four identical polypeptide chains. Eight of these residues are buried inside theExpand
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Thermostable Aminoacylase from Bacillus thermoglucosidius: Purification and Characterization
We studied the distribution of aminoacylase, an enzyme catalyzing the hydrolysis of N-acylamino acids, in thermophilic bacteria, and found Bacillus thermoglucosidius DSM 2542 to be the best producerExpand
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Properties of α-Amino-ε-caprolactam Racemase from Achromobacter obae
α-Amino-e-caprolactam racemase, which occurs in the cytoplasmic fraction of Achromobacter obae, has been purified to homogeneity. It has a monomeric structure with a molecular weight of approximatelyExpand
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In Vitro Study on Contribution of Oxidative Metabolism of Isolated Rabbit Heart Mitochondria to Myocardial Reperfusion Injury
The present study was performed to clarify oxygen-induced damage following myocardial reperfusion, using three mitochondrial preparations from isolated rabbit hearts (non-ischemic hearts and thoseExpand
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A versatile bacterial enzyme: l-methionine γ-lyase
Abstract The properties and application of l -methionine γ-lyase [methioninase, l -methionine methanethiol-lyase (deaminating), EC 4.4.1.11], a pyridoxal 5′-phosphate enzyme, purified fromExpand
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