Hidehiko Ochiai

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A double immunocytochemical method combining the preembedding PAP technique and the postembedding immunogold technique was used to examine interactions between GABAergic and serotonergic neurons in the same tissue sections of the dorsal raphe nucleus of the rat. A large number of immunogold stained GABAergic axon terminals were found to be presynaptic to(More)
In the present paper, the distribution of a neuron-specific phosphoneuroprotein 14 (PNP 14) in cell and tissue was investigated in detail by the immunoblot method using affinity-purified antibody against this protein. The immunoblot of the supernatant fractions of various tissue homogenates of rat clearly demonstrated that PNP 14 was enormously rich in the(More)
Mouse erythrocytes modified by oxidizing agents (ADP/Fe3+, tert-butyl hydroperoxide and phenylhydrazine) were examined for recognition by autologous macrophages (cell adhesion and phagocytosis). Treatment of erythrocytes with ADP/Fe3+ resulted in lipid oxidation but no significant alterations in physical properties, and the treated cells were adherent to(More)
Synaptophysin and synapsin I, the synaptic vesicle-associated proteins, were demonstrated immunocytochemically in nerve terminals of the neurohypophysis of rats. Although both are associated with microvesicles 50-60 nm in diameter, they are not localized on or around the large neurosecretory granules nor the clear vacuoles, 100-200 nm in diameter. These(More)
The noradrenergic innervation of vasopressin (VP) neurons in the paraventricular nucleus (PVN) of the rat was studied ultrastructurally by double-labeling immunocytochemistry combining the preembedding peroxidase-antiperoxidase method for dopamine-beta-hydroxylase (DBH) with the post-embedding immunogold staining method for neurophysin II, the carrier(More)
Adherent cells derived from human palatine tonsils were isolated and cultivated. Exponentially growing adherent cells (TAC) were observed by phase-contrast microscopy and transmission electron microscopy. Immunocytochemical studies were also performed. TAC were composed of relatively monotonous cells with polygonal or spindle shapes and high proliferative(More)
The catecholaminergic innervation of vasopressin (VP) neurons in the paraventricular nucleus (PVN) of the rat was studied at the electron microscopic level by double-labeling immunocytochemistry combining the preembedding peroxidase-antiperoxidase method with the postembedding immunogold staining method. Tyrosine hydroxylase-like immunoreactive nerve(More)
The neuropeptide Y (NPY) immunoreactive synaptic input to neurons containing neurophysin II (NP II), the carrier protein of vasopressin (VP), was observed in the paraventricular nucleus (PVN) of the rat hypothalamus by double-labeling immunocytochemistry combining the preembedding peroxidase-antiperoxidase (PAP) method with the postembedding immunogold(More)
Blue-green fluorescent subependymal cells with intraventricular processes were shown by the fluorescent histochemical method to be distributed from the preoptic recess to the infundibular recess of the frog hypothalamus. Electron microscopy revealed at least two types of CSF-contacting subependymal cells, type 1 containing large dense granules (about(More)