Herbert Stekel

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BACKGROUND Human herpes viruses cause a spectrum of diseases that are usually self-limiting but can be reactive during immuno-suppression and may then lead to severe or even life-threatening diseases. The LightCycler technology allows rapid polymerase chain reaction (PCR) including product analysis within a closed system. This approach has been demonstrated(More)
Fourth-generation assays for the simultaneous detection of human immunodeficiency virus (HIV) antigen and antibody that were available on the international market until now have antigen detection modules with relatively poor sensitivity and produce a higher rate of false-positive results than third-generation enzyme immunoassays (EIAs). The new Cobas Core(More)
Early detection of hepatitis C virus (HCV) is an important step in preventing progression to cirrhosis and hepatocellular carcinoma. Serologic assays for anti-hepatitis C (anti-HCV) antibody are valuable first-line tests in the screening and diagnosis of HCV infection. The aim of this multicenter study was to compare the Elecsys® Anti-HCV assay with(More)
In this paper we present results of empirical research work done on the data based identification of estimation models for cancer diagnoses: Based on patients' data records including standard blood parameters, tumor markers, and information about the diagnosis of tumors we have trained mathematical models for estimating cancer diagnoses. Several data based(More)
Objective: To investigate whether the genes encoding Cyclooxygenase-1 and -2 are expressed in thyroid epithelial cells, in vitro.¶Materials and Methods: COX-1/-2 gene expression was examined in the thyroid epithelial cell line Nthy-ori3-1 using semi-quantitative RT-PCR and Western blot analysis. ELISAs were employed to assess whole cell COX-enzyme activity,(More)
BACKGROUND For many diagnostic applications, the specificity and sensitivity of polymerase chain reaction (PCR) is markedly enhanced by applying two rounds of PCR with nested or semi-nested pairs of primers. In two-round PCR protocols on the LightCycler instrument, amplification products must be collected from the capillaries by centrifugation, a procedure(More)
In this paper we present results of empirical research work done on the data based identification of estimation models for cancer diagnoses: Based on patients’ data records including standard blood parameters, tumor markers, and information about the diagnosis of tumors we have trained mathematical models for estimating cancer diagnoses. Several data based(More)
A sequencing assay for detection of mutations conferring resistance to human immunodeficiency virus type 1 (HIV-1) integrase inhibitors raltegravir and elvitegravir was developed using the automated TruGene sequencing system. The assay returned clear sequencing results for samples with >or=500 RNA copies/ml for mutation detection and HIV-1 subtyping across(More)
Standard patient parameters, tumor markers, and tumor diagnosis records are used for identifying prediction models for tumor markers as well as cancer diagnosis predictions. In this paper we present a hybrid clustering and classification approach that first identifies data clusters (using standard patient data and tumor markers) and then learns prediction(More)