Herbert Schmitt

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We developed a photodynamic method to inactivate viruses in human fresh plasma. Single plasma bags were illuminated with visible light in the presence of low doses of phenothiazine dyes like methylene blue or toluidine blue. By this treatment the infectivity of different enveloped viruses including the causative agent of AIDS, HIV-1, was completely(More)
BACKGROUND Lumbar disc bulging has been determined with different methods in the past. Reported methods of bulging assessment were limited to a direct physical contact, were two-dimensional and were time-consuming. Assessing the three-dimensional contour of a biological object under load would imply that the tissue would creep and therefore changes its(More)
Second generation enzyme immunoassays (EIA-2) for antibodies to hepatitis C virus (anti-HCV) have a higher specificity and sensitivity than first generation enzyme immunoassays (EIA-1). We studied how many anti-HCV-positive blood donors were missed by the EIA-1, how many were false positive, how false-positive donors should be dealt with and how the results(More)
BACKGROUND Clinically oriented and easy to handle animal models are urgently needed to test pharmacologic treatment of cartilage trauma to reduce the resulting tissue damage by chondrocyte apoptosis and induction of matrix-degrading enzymes. AIM To develop a biomechanically defined cartilage trauma model. MATERIAL AND METHODS We constructed a novel(More)
To examine the consistency and comparability of anti-hepatitis B core antigen (anti-HBcAg) assays, four blood donation centers of the Red Cross in the Federal Republic of Germany tested 4,080 unselected blood donors with six different tests in parallel. Confirmation testing of reactive samples was done in the National Reference Center for Viral Hepatitis.(More)
BACKGROUND Second-generation enzyme immunoassays (EIA-2) for antibodies to hepatitis C virus (anti-HCV) have an improved specificity and sensitivity compared to first-generation enzyme immunoassays (EIA-1). Therefore the question arises how many anti-HCV-positive blood donors were missed by the EIA-1, how many were false positive, how false-positive donors(More)
Photodynamic virus inactivation of human fresh plasma is achieved by illuminating single units of plasma in their plastic containers with light from fluorescent tubes in the presence of the phenothiazine dye methylene blue. The effective dye concentration is 1 microM, i.e. approx. 300 micrograms per litre of plasma. Under the conditions used, all enveloped(More)
A photodynamic procedure to inactivate viruses in fresh plasma for therapeutical use is carried out by illuminating single units of plasma with visible light in the presence of the phenothiazine dye methylene blue. The blood bag systems of all common suppliers can be used for this purpose. Photodynamic treatment only moderately influences the activities of(More)