Henryk Kołoczek

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In search of the optimal way to reduce the hazards of environmental contamination by formaldehyde (FD) and methanol the use of unconventional yeasts is proposed as exemplified by the methylotrophic yeast Hansenula polymorpha. In a very simplified environment of a model wastewater solution, H. polymorpha cells were able to grow on, and metabolize(More)
A yeast isolate revealing unique enzymatic activities and substrate-dependent polymorphism was obtained from autochthonous microflora of soil heavily polluted with oily slurries. By means of standard yeast identification procedures the strain was identified as Trichosporon cutaneum. Further molecular PCR product analyses of ribosomal DNA confirmed the(More)
Flavinogenic yeast overproduce riboflavin (RF) in iron-deprived media. In optimal growth media supplemented with Fe, hexavalent chromium 'Cr (VI)' treatment led to elevated RF synthesis in all cases of 37 flavinogenic strains studied. The level of RF production exceeded the rate observed at iron-deficient conditions. At sublethal Cr concentrations the RF(More)
The application of methylotrophic yeast Hansenula polymorpha to the treatment of methanol and formaldehyde-containing wastewater was experimentally verified. Avariety of real wastewater samples originating from chemical industry effluent were examined. The yeast cell culture could grow in the wastewater environment, revealing low trophic requirements and a(More)
In biological systems, metalloporphyrins play a central role in energy and electron transfer process. Our aim is to understand the influence of ligands and iron coordination of ironporphyrin on the electron transfer. The lyophilized ironporphyrin, enriched in 57Fe up to 90% has been studied by Mössbauer spectroscopy between 2.8 and 313 K. Above room(More)
Callitriche cophocarpa (water-starwort)--aquatic widespread macrophyte--was found to be an excellent chromium accumulator. The plants were exposed to various chromium(VI) concentration ranging from 50 to 700 microM in a hydroponic culture up to ca. 3 weeks. Physiological conditions of shoots were monitored via measuring potential photosynthesis quantum(More)
Chromium in the sixth oxidation state may easily penetrate cellular membranes via non-specific sulfate transporters due to its tetrahedral symmetry (high similarity to SO4(2-) and HPO4(2-)). This feature makes chromium a toxic and hazardous pollutant responsible for the deterioration of midland water quality. The aim of the study was to evaluate the(More)
Sulforaphane (SFN) is an isothiocyanate present in cruciferous vegetables, which has been shown to exert an anti-cancer effect when tested in vitro and in vivo. The anti-cancer effects of SFN encompass induction of cytoprotective autophagy; therefore, the present study aimed to determine whether the chemopreventive activity of SFN may be potentiated by(More)
The human alpha 1-proteinase inhibitor (alpha 1-PI) and its reactive site modified form (alpha 1-PI*) have been examined using the fluorescence quenching resolved spectra method. The red-edge excitation measurements were applied for the study of structural differences between these forms. The crystallographic data of alpha 1-PI* structure have shown that(More)
Nuclear magnetic relaxation studies have been performed on thiosulfate sulfurtransferase (EC 2.8.1.1) and hexokinase (EC 2.7.1.1). Observation of proton spin-lattice relaxation times T1 indicates that structural transitions occur in these enzymes in the range 0-40 degrees C and that there are different temperature-dependent forms of thiosulfate(More)