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Aging and physiological androgen decay leads to structural changes in corpus cavernosum (CC) that associate with erectile function impairment. There is evidence that such changes relate to nitric oxide (NO) bioavailability, an endothelial compound produced by the action of endothelial NO synthase (eNOS), and is regulated by sirtuin-1 (Sirt1), a(More)
High-fat (HF) diet regular intake along life highly contributes to vascular dysfunction and to an increment in prevalence of metabolic syndrome (MetS) and erectile dysfunction (ED), a surrogate symptom of occult vascular disease, in the elderly. However, little is known about the effects of energy restriction (ER) alone/or after an HF-feeding period. We(More)
OBJECTIVE The aim of the study was to construct gestational age-based reference ranges for the uterine artery (UtA) mean pulsatility (PI) and resistance (RI) indices from 6 to 10 weeks of pregnancy. METHOD A prospective, cross-sectional, observational study was carried out in 312 singleton pregnancies with gestational age ranging from 6 to 10 weeks. UtAs(More)
Human diploid fibroblasts (HDFs) exposed to subcytotoxic concentrations of oxidative or stressful agents, such as hydrogen peroxide, tert-butylhydroperoxide, or ethanol, undergo stress-induced premature senescence (SIPS). This condition is characterized by the appearance of replicative senescence biomarkers such as irreversible growth arrest, increase in(More)
Oxidative stress, OS, has been associated to a variety of phenomena as cancer progression, neurodegeneration and ageing itself. At a molecular level, OS leads to protein carbonylation, a non-enzymatic irreversible event and common feature of aged cells. Carbonylated proteins are dysfunctional and can accumulate, in the form of protein aggregates that alter(More)
The aging process is characterized by progressive accumulation of damaged biomolecules in the endoplasmic reticulum, as result of increased oxidative stress accompanying cellular senescence. In agreement, we hypothesized that WI-38 human cellular models of replicative senescence and stress-induced premature senescence (SIPS) induced by hydrogen peroxide(More)
Melanocortin 5 receptor (MC5R) is a G protein coupled receptor (GPCR) with high affinity for alpha-melanocyte-stimulating hormone (alpha-MSH). To unravel some of the downstream cell-signaling pathways activated by this receptor, HEK293 cells were transiently and stably transfected with a vector encoding green fluorescent protein (GFP)-tagged MC5R. In these(More)
Protein carbonylation is a common feature in cells exposed to oxidants, leading to protein dysfunction and protein aggregates. Actin, which is involved in manifold cellular processes, is a sensitive target protein to this oxidative modification. T-cell proteins have been widely described to be sensitive targets to oxidative modifications. The aim of this(More)
The Melanocortin 5 receptor (MC5R) is a G-protein coupled receptor (GPCR) that exhibits high affinity for α-MSH. Here we present evidence for MC5R-GFP internalization and subsequent recycling to cell surface, in α-MSH-stimulated HeLa cells. This melanocortin induces a biphasic activation of ERK1/2 with an early peak at 15min, a G(i)-protein driven,(More)
Macrophages are present throughout the adrenal cortex, particularly in the deeper layers. They locate next to parenchyma cells, which secrete glucocorticoids under the regulation of the pituitary adrenocorticotropic hormone (ACTH). Blockade of ACTH secretion is followed by adrenocortical cell atrophy and apoptosis, an effect likely to cause an increase in(More)