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Administered intravenously to rats, CDPcholine, significantly increases the level and the synthesis rate of dopamine, and the level of tyrosine in the corpus striatum (maximum effect for 50 mg/kg, one hour after administration). CDPcholine decreases the level of serotonin and tryptophan and the synthesis rate of serotonin in the midbrain + hypothalamus and(More)
In order to investigate the mechanisms involved in some brain disorders at the membrane level, we studied the kinetics and biochemical properties of brain CTP:choline-phosphate cytidylyltransferase (EC, the rate-limiting enzyme of the two-step biosynthesis of phosphatidylcholine. This enzyme catalyzes the biosynthesis of CDPcholine from choline(More)
The concentrations of S-adenosyl-L-methionine (SAM), S-adenosyl-L-homocysteine (SAH), and adenosine (Ado) were determined in whole brain and rat brain regions by HPLC. The whole brain contains, respectively, 22 nmol, 1 nmol, and 65 nmol of SAM, SAH, and Ado per g of wet tissue. Their distribution indicated that SAM and SAH levels are highest in brainstem,(More)
The effect of low-level microwave exposure, 2,450 MHz, at a power density of 1 mW/cm2 and specific absorption rate of 1.2 mW/g, continuous waves (CW) or pulsed waves (PW), 2.5 h/day, 6 sessions/week until death (up to 690 h of irradiation), has been studied in black C57/6J mice with B16 melanoma. The results show that no significant effects are observed on(More)
Tissue distribution, metabolism and excretion of14C-S-adenosyl-L-homocyteine were investigated in rat after oral and intraperitoneal administration. The radioactivity was distributed rapidly to the tissues. The urine was the major route of excretion: respectively 32% and 52% of radioactivity were excreted in 48 h urine after oral and intraperitoneal(More)
A series of synthetic pentasubstituted analogs of quercetin were evaluated for their ability to inhibit the various phosphodiesterase isoforms resolved from rat brain cytosol by isoelectric focusing. All the tested compounds were more potent in inhibiting the calcium plus calmodulin-independent isoforms than the dependent ones. Out of the two(More)
Added to a striatal synaptosomal homogenate of rat brain, CDP-choline 10−4 M inhibits the uptake of norepinephrine (NE), dopamine (DA) and serotonin (5 HT) in a competitive fashion and enhances the uptake of tyrosine and tryptophan; administered to animals, CDP-choline (50 mg/kg/l h/i.v.) inhibits only the in vitro uptake of DA but enhances the uptake of(More)
Intraperitoneal coadministration of adenosine and L-homocysteine markedly increased S-adenosyl-L-homocysteine in whole mouse brain, but further investigations showed that this elevation could also be produced following administration of L-homocysteine alone. The noted increase was maximal (+1325%) 10 min after treatment, remaining at about this level for(More)
When synaptosomes from rat brain were incubated with S-adenosylmethionine (10(-3) M), noradrenaline uptake and KCl-stimulated release were decreased. These effects were dependent on MgCl2, temperature, and incubation time. We have investigated the enzymatic methylation of phosphatidylethanolamine to give phosphatidylcholine.
L-Homocysteine intraperitoneal injections to the rat induced 50-70% inhibition of phosphatidyl-N-methyltransferase activity. A 30-40% increase of muscimol (a gamma-aminobutyric acid (GABA) agonist) binding was observed; Scatchard plot analysis showed an increase of the number of sites. [3H]Diazepam binding was not modified.