Henny van Rooy

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Invasion by MO4 mouse fibrosarcoma cells into fragments of embryonic chick heart or lung in organ culture was studied histologically and ultrastructurally at various temperatures between 12 and 40°C. Invasion was absent for at least 7 days at or below temperatures of 29°C. Invasion was invariably observed at or above 30·5°C. Differences in invasion between(More)
Invasion of malignant MO4 cells into embryonic chick heart fragments in an organ culture assay was arrested for at least 7 days when the temperature was lowered to 28°C. Prolonged culturing of MO4 cells at 28°C on tissue culture substrates showed no recuperation of fucose incorporation into cell surface glycopeptides. However, invasion was restored after 10(More)
Long-term mouse urothelial cell cultures were routinely established from explants of neonatal mouse bladders. Foci of proliferating cells could be observed one week after the initiation of the explant cultures. These persisted throughout the culture period and up to one year. Expression of keratin proteins confirmed the epithelial nature of the cultured(More)
The effect of alterations in cell surface carbohydrates on invasion of mouse and rat cells into embryonic chick heart fragments in organ culture was studied. Matching pairs of malignant and nonmalignant cells, includ ing all categories of carcinogenic induction (i.e., viral, chemical, or oncogenic), were compared for their alterations in cell surface(More)
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