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Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing
The purification and characterization of an EED-EZH2 complex, the human counterpart of the Drosophila ESC-E(Z) complex, is reported, and it is demonstrated that the complex specifically methylates nucleosomal histone H3 at lysine 27 (H3-K27).
Role of histone H2A ubiquitination in Polycomb silencing
The purification and functional characterization of an E3 ubiquitin ligase complex that is specific for histone H2A is reported, and it is linked to Polycomb silencing, which is important in regulating chromatin dynamics and transcription.
Role of Histone H3 Lysine 27 Methylation in X Inactivation
It is demonstrated that transient recruitment of the Eed-Ezh2 complex to the inactive X chromosome (Xi) occurs during initiation of X inactivation in both extraembryonic and embryonic cells and is accompanied by H3-K27 methylation.
Methylation of Histone H4 at Arginine 3 Facilitating Transcriptional Activation by Nuclear Hormone Receptor
A mutation in theS-adenosyl-l-methionine–binding site of PRMT1 substantially crippled its nuclear receptor coactivator activity and indicates that Arg 3 methylation plays an important role in transcriptional regulation.
Methylation of H3-Lysine 79 Is Mediated by a New Family of HMTases without a SET Domain
Lysine methylation within the globular domain of histone H3 by Dot1 is important for telomeric silencing and Sir protein association.
It is reported that lysine (Lys) 79 of histone H3, which resides in the globular domain, is methylated in eukaryotic organisms and indicates that histone modifications in the core globulardomain have important biological functions.
Histone H3 and H4 ubiquitylation by the CUL4-DDB-ROC1 ubiquitin ligase facilitates cellular response to DNA damage.
mAM facilitates conversion by ESET of dimethyl to trimethyl lysine 9 of histone H3 to cause transcriptional repression.
Regulation of cell cycle progression and gene expression by H2A deubiquitination
It is demonstrated that H2A deubiquitinase is critically involved in cell cycle progression and gene expression and that blocking the function of Ubp-M results in defective posterior development in Xenopus laevis.