Helen Stoeber

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In this study, we investigated the interaction of 19 benign strains of lactic acid bacteria (LAB), bifidobacteria and staphylococci with enterohemorrhagic Escherichia coli (EHEC) strains of different serotypes and virulence gene spectrum in a HT29 cell culture infection model. As markers of infection, the secretion of interleukin 8 (IL-8) and the activation(More)
Seventy-five food-associated Shiga toxin-producing Escherichia coli (STEC) strains were analyzed by molecular and phylogenetic methods to describe their pathogenic potential. The presence of the locus of proteolysis activity (LPA), the chromosomal pathogenicity island (PAI) PAI ICL3, and the autotransporter-encoding gene sabA was examined by PCR.(More)
The open reading frames of subAB genes and their flanking regions of 18 food-borne Shiga toxin-producing E. coli (STEC) strains were analyzed. All but one subAB open reading frames (ORF) were complete in all STEC strains. The subAB 1 genes of nine STEC strains were located on large plasmids. The subAB 2 allele (here designated subAB 2-1), which was recently(More)
Table 1 Designations, targets, and positions of primers for PCR analysis and Southern blot hybridization Primer Target Sequence (5′ – 3′) GenBank accession number Reference saaDF saa 5′-CGT GAT GAA CAG GCT ATT GC-3′ AF399919 [26] saaDR saa 5′-ATG GAC ATG CCT GTG GCA AC-3′ AF399919 [26] subAB-V-for subAB 5′-CTT CCC TCA TTG CCT CAC G-3′ AY258503 This study(More)
In this study, the 1938bp open reading frame z1466, which is encoded directly downstream the Shiga toxin 2a (Stx2a) operon in E. coli O157:H7 phage 933W was cloned and expressed recombinantly. Purification with Ni-NTA agarose beads with subsequent SDS-PAGE revealed a 68kDa protein, designated 933Wp42-His. Analysis of 933Wp42-His demonstrated an esterase(More)
Enterohemorrhagic Escherichia coli (EHEC) are food-borne pathogens implicated in large outbreaks and sporadic cases of bloody diarrhea and the hemolytic uremic syndrome. The main reservoir of EHEC is the intestinal tract of ruminants, in particular cattle. Feces containing these bacteria may act as a source of contamination for the environment and(More)
Table 1 Designations, targets, and positions of primers for PCR analysis and Southern blot hybridization Primer Target Sequence (5′ – 3′) GenBank accession number Reference saaDF saa 5′-CGT GAT GAA CAG GCT ATT GC-3′ AF399919 [26] saaDR saa 5′-ATG GAC ATG CCT GTG GCA AC-3′ AF399919 [26] subAB-V-for subAB 5′-CTT CCC TCA TTG CCT CAC G-3′ AY258503 This study(More)
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