Learn More
Seventy-five food-associated Shiga toxin-producing Escherichia coli (STEC) strains were analyzed by molecular and phylogenetic methods to describe their pathogenic potential. The presence of the locus of proteolysis activity (LPA), the chromosomal pathogenicity island (PAI) PAI ICL3, and the autotransporter-encoding gene sabA was examined by PCR.(More)
The open reading frames of subAB genes and their flanking regions of 18 food-borne Shiga toxin-producing E. coli (STEC) strains were analyzed. All but one subAB open reading frames (ORF) were complete in all STEC strains. The subAB 1 genes of nine STEC strains were located on large plasmids. The subAB 2 allele (here designated subAB 2-1), which was recently(More)
In this study, we investigated the interaction of 19 benign strains of lactic acid bacteria (LAB), bifidobacteria and staphylococci with enterohemorrhagic Escherichia coli (EHEC) strains of different serotypes and virulence gene spectrum in a HT29 cell culture infection model. As markers of infection, the secretion of interleukin 8 (IL-8) and the activation(More)
Correction: Molecular analysis of subtilase cytotoxin genes of food-borne Shiga toxin-producing Escherichia coli reveals a new allelic subAB variant After publication of this work [1], we noticed that the sequences of primers subA-L and subAB5'-OEP in Table two (Table 1 here) are not correct. We therefore provide the correct primer sequences in a new Table(More)
In this study, the 1938bp open reading frame z1466, which is encoded directly downstream the Shiga toxin 2a (Stx2a) operon in E. coli O157:H7 phage 933W was cloned and expressed recombinantly. Purification with Ni-NTA agarose beads with subsequent SDS-PAGE revealed a 68kDa protein, designated 933Wp42-His. Analysis of 933Wp42-His demonstrated an esterase(More)
Correction: Molecular analysis of subtilase cytotoxin genes of food-borne Shiga toxin-producing Escherichia coli reveals a new allelic subAB variant After publication of this work [1], we noticed that the sequences of primers subA-L and subAB5'-OEP in Table two (Table 1 here) are not correct. We therefore provide the correct primer sequences in a new Table(More)
  • 1