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Disorders of wound healing characterized by impaired or delayed re-epithelialization are a serious medical problem. These conditions affect many tissues, are painful, and are difficult to treat. In this study, using cornea as a model, we demonstrate for the first time the importance of carbohydrate-binding proteins galectins-3 and -7 in re-epithelialization(More)
PURPOSE To identify differentially expressed genes in healing mouse corneas by using cDNA microarrays. METHODS Transepithelial excimer laser ablations were performed on mouse corneas, and the wounds were allowed to heal partially in vivo for 18 to 22 hours. Total RNA was isolated from both normal and healing corneas and was used for synthesis of cDNA(More)
Although laser in situ keratomileusis (LASIK) enjoys a high success rate, postoperative residual or induced astigmatism may limit uncorrected visual acuity and cause starbursts and glare at night. Irregular astigmatism can also cause loss of best-corrected visual acuity, monocular diplopia, and ghosting of images. Astigmatism may be measured by keratometry(More)
OBJECTIVE To assess the role of a carbohydrate-binding protein, galectin-7, in reepithelialization of corneal wounds. METHODS Transepithelial excimer laser ablations were performed on mouse corneas, and the wounds were allowed to partially heal in vivo for 18 to 22 hours. At the end of the healing period, expression levels of galectin-7 messenger RNA and(More)
OBJECTIVE To determine whether tears of healthy individuals provide protection against Acanthamoeba-induced cytopathic effect (CPE) in vitro. METHODS Acanthamoebae were added to confluent cultures of corneal epithelium in 24-well plates, and co-cultures were incubated overnight in a serum-free medium containing varying amounts of tears or immunoglobulin A(More)
Elevated soluble interleukin-2 receptor levels (sIL-2R), a measure of T cell activation, have been used as a serum marker for early rejection in solid organ transplant patients. In this preliminary study, we measured sequential sIL-2R levels of 53 otherwise immunologically normal corneal transplant recipients. Eleven of these transplants rejected; sIL-2R(More)
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