Helen Gu

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To investigate the role of turns in protein folding, we have characterized the effects of combinatorial and site-directed mutations in the two beta-turns of peptostreptococcal protein L on folding thermodynamics and kinetics. Sequences of folded variants recovered from combinatorial libraries using a phase display selection method were considerably more(More)
We use both combinatorial and site-directed mutagenesis to explore the consequences of surface hydrophobic substitutions for the folding of two small single domain proteins, the src SH3 domain, and the IgG binding domain of Peptostreptococcal protein L. We find that in almost every case, destabilizing surface hydrophobic substitutions have much larger(More)
We have developed a phage display system that provides a means to select variants of the IgG binding domain of peptostreptococcal protein L that fold from large combinatorial libraries. The premise underlying the selection scheme is that binding of protein L to IgG requires that the protein be properly folded. Using a combination of molecular biological and(More)
Our work is motivated by the desire to develop methods to comprehend large vocabularies and large schemas of Object-Oriented Databases. The ability of a user of a database participating in a federated system to retrieve information from the other database systems will be greatly enhanced by acquiring a better comprehension of these systems. We are trying to(More)
A software system interacts with third-party libraries through various Application Program Interfaces (APIs). Using these APIs correctly often needs to follow certain programming rules, i.e., API specifications. API specifications specify the required checks (on API input parameters and return values) and other APIs to be invoked before (preconditions) and(More)
Epothilones are natural macrolides displaying potent antiproliferative properties against various cell lines and capable to bind tubulin and acting as microtubule-stabilizing agents like taxoids. We intended to isolate and characterize epothilone metabolites and identify enzymes implicated in the biotransformation process. In the presence of NADPH, liver(More)
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