Heinz Kohler

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Conceptually, one antibody could be directed against a combining site structure of another antibody; thus, the two antibodies would combine with each other and might be considered at least partially complementary. Since the combining regions of antigen receptors on B lymphocytes are apparently identical with the combining regions of the antibodies that the(More)
A hybridoma antibody (11E7-1) was isolated from a myeloma fusion with nu/nu BALB/c immunized against the T15 idiotype. This IgM antibody exhibited a dual specificity, binding both to PC and to anti-PC antibodies from two idiotype families. Binding to PC and anti-PC antibodies are completely inhibited by PC analogs. Furthermore, the hybridoma antibody binds(More)
We have previously described (1-3) an IgM antibody that binds to PC, expresses the T15 idiotype, and binds also to itself or T15 if insolubilized. Because of the simultaneous presence of complementary idiotopes and paratopes this type of antibody has been termed autobody. The self binding involves the antigen-binding site because the F(ab')2 fragment of(More)
In this study T helper cells that recognize idiotypes as carriers for a hapten-specific B cell response were analyzed under limiting dilution conditions. T helper cells, induced by phosphorylcholine-hemocyanin (PC-Hy) priming, recognize trinitrophenylated TEPC-15 and MOPC-167 (TNP-T15, TNP-167) equally well. Limiting dilution analysis indicates identical(More)
A basic tenet of antibody-based immunity is their specificity to antigenic determinates from foreign pathogen products to abnormal cellular components such as in cancer. However, an antibody has the potential to bind to more than one determinate, be it an antigen or another antibody. These observations led to the idiotype network theory (INT) to explain(More)
To estimate the minimal structural requirements for cross-reaction of idiotypic determinants, we determined the capacity of monoclonal antibodies specific for the idiotype of the phosphorylcholine (PC)-binding myeloma protein TEPC-15 for cross-reactivities with the PC-binding, acute-phase protein C-reactive protein (CRP) and the hemagglutinin from the(More)
Previously, we have demonstrated the induction of T helper cells that recognize idiotype by antigen (19), idiotype (20), and antiidiotype (12). The T cell population has been characterized and found to recognize both the T15 and M167 myeloma proteins, which share PC binding specificity but differ in idiotypic specificities. In the present work, we used(More)
Live SIV vaccine correlate of protection: immune complex-inhibitory Fc receptor interactions that reduce target cell availability" (2014). Virology Papers. Paper 313. Abstract Principles to guide design of an effective vaccine against HIV are greatly needed, particularly to protect women in the pandemic's epicentre in Africa. We have been seeking these(More)
The effects of response probability and feedback predictability on the human Bereitschaftspotential (BP) were examined in a probability-matching paradigm. The subjects were to guess, by pressing one of two buttons, which of two possible feedback lights could be lighted. The a priori probabilities (30%-70%) of the feedback were known to the subjects. This(More)
The homophilic potential emerges as an important biological principle to boost the potency of immunoglobulins. Since homophilic antibodies in human and mouse sera exist prior environmental exposure, they are part of the natural antibody repertoire. Nevertheless, hemophilic properties are also identified in induced antibody repertoire. The use of(More)