Due to photo-toxicity, fluorescence microscopy imaging is a trade-off between signal to noise ratio, total observation time and spatio-temporal resolution. We propose a new and simple method to quantify the signal-dependent and the non-signal-dependent components of the noise from a single fluorescence microscopy image. No reference image is required and… (More)
Probe photobleaching and a specimen's sensitivity to phototoxicity severely limit the number of possible excitation cycles in time-lapse fluorescent microscopy experiments. Consequently, when a study of cellular processes requires measurements over hours or days, temporal resolution is limited, and spontaneous or rapid events may be missed, thus limiting… (More)
Heinrich J Huber (firstname.lastname@example.org) Maike A Laussmann (email@example.com) Jochen HM Prehn (firstname.lastname@example.org) Markus Rehm (email@example.com) Like all articles in BMC journals, this peer-reviewed article was published immediately upon acceptance. It can be downloaded, printed and distributed freely for any purposes (see copyright notice below). which permits… (More)
This document is the Reference Information Model (RIM) of the AmbieSense Project. It provides the project's vocabulary and a consistent description the AmbieSense technology and how it can be applied. This is done by describing the overall system and architecture, the AmbieSense core technology, and four example applications.