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Due to photo-toxicity, fluorescence microscopy imaging is a trade-off between signal to noise ratio, total observation time and spatio-temporal resolution. We propose a new and simple method to quantify the signal-dependent and the non-signal-dependent components of the noise from a single fluorescence microscopy image. No reference image is required and(More)
Probe photobleaching and a specimen's sensitivity to phototoxicity severely limit the number of possible excitation cycles in time-lapse fluorescent microscopy experiments. Consequently, when a study of cellular processes requires measurements over hours or days, temporal resolution is limited, and spontaneous or rapid events may be missed, thus limiting(More)
Heinrich J Huber (heinhuber@rcsi.ie) Maike A Laussmann (mlaussmann@rcsi.ie) Jochen HM Prehn (prehn@rcsi.ie) Markus Rehm (mrehm@rcsi.ie) Like all articles in BMC journals, this peer-reviewed article was published immediately upon acceptance. It can be downloaded, printed and distributed freely for any purposes (see copyright notice below). which permits(More)
  • Stein L Tomassen, Till C Lech, Juergen Pollich, Frank Sembowski, Yellowmap, Børge Haugset +16 others
  • 2003
This document is the Reference Information Model (RIM) of the AmbieSense Project. It provides the project's vocabulary and a consistent description the AmbieSense technology and how it can be applied. This is done by describing the overall system and architecture, the AmbieSense core technology, and four example applications.
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