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Intracellular localization and transcriptional regulation of tumor necrosis factor (TNF) receptor-associated factor 4 (TRAF4).
TLDR
In primary T-cells and Jurkat cells stimulated with the NF-kappaB inducers TNF or phorbol 12-myristate 13-acetate (PMA), TRAF4-mRNA was rapidly up-regulated and activated, suggesting that activation of theNF- kappaB pathway is involved in up-regulation of TRAf4 in T- cells. Expand
Tumor necrosis factor receptor-associated factor (TRAF) 1 regulates CD40-induced TRAF2-mediated NF-kappaB activation.
TLDR
It is proposed that the stoichiometry of TRAF1-TRAF2 heteromeric complexes determines their capability to mediate CD40 signaling but has no major effect on TNF signaling. Expand
Tumor Necrosis Factor Receptor-associated Factor (TRAF) 1 Regulates CD40-induced TRAF2-mediated NF-κB Activation*
TLDR
It is proposed that the stoichiometry of TRAF1-TRAF2 heteromeric complexes determines their capability to mediate CD40 signaling but has no major effect on TNF signaling. Expand
Simultaneous detection of intracellular target and off‐target binding of small molecule cancer drugs at nanomolar concentrations
TLDR
A combination of fluorescence correlation spectroscopy (FCS) and fluorescence cross‐correlation spectroscope (FCCS) is presented as a solution to the problem of off‐target binding and membrane permeability in vitro assays. Expand
The intracellular pharmacokinetics of terminally capped peptides.
TLDR
Analysis of the structure-activity relationship of peptides with respect to intracellular residence time and proteolytic breakdown provides a straightforward analytical access to a better understanding of the principles of peptide stability inside cells and will therefore greatly assist the development of bioactive peptides. Expand
The in vitro biological activity of the HLA-DR-binding clinical IgG4 antibody 1D09C3 is a consequence of the disruption of cell aggregates and can be abrogated by Fab arm exchange.
TLDR
The results indicate that the activity of 1D09C3 in vitro may have been a consequence of assay design rather than an ability to induce HLA-DR-dependent cell death, and this antibody undergoes Fab arm exchange in the presence of IgG4. Expand
Coupling to Polymeric Scaffolds Stabilizes Biofunctional Peptides for Intracellular Applications
TLDR
It is demonstrated that coupling to N-hydroxypropyl methacrylamide (HPMA) copolymer greatly enhances the activity of apoptosis-inducing peptides inside cells, revealing proteolytic protection as the basis for higher activity. Expand
A Quantitative Assessment of Costimulation and Phosphatase Activity on Microclusters in Early T Cell Signaling
TLDR
The role of costimulation and a specific phosphotyrosine phosphatase in cluster formation is addressed by including a SHP2 knock-down strain in this system and enables a clear distinction between factors determining the number of microclusters and those that act on these signaling platforms. Expand