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We describe the high resolution structure of the large ribosomal subunit from Deinococcus radiodurans (D50S), a gram-positive mesophile suitable for binding of antibiotics and functionally relevant ligands. The over-all structure of D50S is similar to that from the archae bacterium Haloarcula marismortui (H50S); however, a detailed comparison revealed(More)
The small ribosomal subunit performs the decoding of genetic information during translation. The structure of that from Thermus thermophilus shows that the decoding center, which positions mRNA and three tRNAs, is constructed entirely of RNA. The entrance to the mRNA channel will encircle the message when a latch-like contact closes and contributes to(More)
copy (cryo-EM) have also revealed new features of ribo-somal particles and their complexes (Gabashvili et al., 1999b, 2000; Stark et al., 2000). In parallel, the structures of several ribosomal protein–RNA complexes have been determined at high resolution (Conn et al. Structure The small ribosomal subunit from T. thermophilus 22603 Hamburg contains 19(More)
Preliminary electron density maps of the large and the small ribosomal particles from halophilic and thermophilic sources, phased by the isomorphous replacement method, have been constructed at intermediate resolution. These maps contain features comparable in size with what is expected for the corresponding particles, and their packing arrangements are in(More)
Crystals, diffracting best to around 3 A, have been grown from intact large and small ribosomal subunits. The bright synchrotron radiation necessary for the collection of the higher-resolution X-ray diffraction data introduces significant decay even at cryo temperatures. Nevertheless, owing to the reasonable isomorphism of the recently improved crystals of(More)
BACKGROUND The bacterial ribosome is a primary target of several classes of antibiotics. Investigation of the structure of the ribosomal subunits in complex with different antibiotics can reveal the mode of inhibition of ribosomal protein synthesis. Analysis of the interactions between antibiotics and the ribosome permits investigation of the specific(More)
Despite the appearance of bacterial strains resistant to all clinical antibiotics, including vancomycin (the 'last resort'), development of new antimicrobial agents has slowed during recent decades. To aid design of new antibiotics, we must develop a detailed understanding of the mechanisms of antibiotic action and antibiotic resistance. Several classes of(More)
Selection of microporous hydrophobic membranes for use in gas/liquid contactors: An experimental approach. Preparation of highly asymmetric hollow fiber membranes from poly(ether imide) by a modified dry-wet phase inversion technique using a triple spinneret. Influence of process parameters on the corrosion properties of electrolytic conversion plasma(More)
An electron density map of the large ribosomal subunit from Bacillus stearothermophilus was obtained at 26 A resolution by single isomorphous replacement (SIR) from a derivative formed by specific quantitative labeling with a dense undecagold cluster. For derivatization, a monofunctional reagent of this cluster was bound to a sulfhydryl group of a purified(More)
Automation of wet chemical analytical procedures, particularly titration, photometry and chromatography, have wide application in industrial laboratories. Very often some sample pretreatment must be carried out to transfer the sample into a suitable state for the measurement or chromatographic process. In industrial analysis, samples are very often solids(More)
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