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BACKGROUND Tomato yellow leaf curl virus (TYLCV) is a member of the family Geminiviridae, genus Begomovirus. To test the infectivity of TYLCV in tomato plants, an improved protocol for inoculation of in vitro-cultured tomato plants was developed. RESULTS A TYLCV isolate was cloned, sequenced and used to construct a 1.8-mer infectious clone. Three weeks(More)
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