Heather Stark

Learn More
In conversation, two people inevitably know different amounts about the topic of discussion, yet to make their references understood, they need to draw on knowledge and beliefs that they share. An expert and a novice talking with each other, therefore, must assess each other's expertise and accommodate to their differences. They do this in part, it is(More)
10 tumor specimens were processed according to a modification of the AMeX method, which allows for paraffin embedding plus immunostaining of the slices. We used this method to determine the growth fraction of tumors using the monoclonal antibody KI-67. Results were essentially the same as those obtained when studying frozen sections. The quality of the(More)
Exercise-induced decreases in the (1)H transverse relaxation rate (R(2)) of muscle have been well documented, but the mechanism remains unclear. In this study, the hypothesis was tested that R(2) decreases could be explained by pH decreases and apparent intracellular volume (V(i)') increases. (31)P and (1)H spectroscopy, biexponential R(2) analysis, and(More)
A procedure utilizing the specific inhibition of calf thymus DNA-directed RNA polymerase B has been applied to the quantitation of amanitins. This procedure has permitted the accurate quantitation of alpha-amanitin in amounts as low as 0.05 nanogram, a sensitivity 2000-fold greater than chemical detection methods used following tlc. Analysis of extracts(More)
We present "molecular threading", a surface independent tip-based method for stretching and depositing single and double-stranded DNA molecules. DNA is stretched into air at a liquid-air interface, and can be subsequently deposited onto a dry substrate isolated from solution. The design of an apparatus used for molecular threading is presented, and(More)
The appearance of new peaks in the 7.7-8.6 and 6.8-7.4 ppm regions of the postexercise (1)H spectrum of frog muscle is reported. These new peaks result from the splitting of single pre-exercise carnosine C-2 and C-4 peaks into two peaks, representing the intracellular pH (pH(I)) of oxidative and glycolytic fibers. The following data support this conclusion:(More)
Semiotics also includes the general principles that underlie all sign systems. It is thus more comprehensive than linguistics--very much more, because there is a semiotic dimension to practically every human artifact. (Indeed, since we can 'read in' meanings to natural events, e.g., as in 'the lack of rain shows God's displeasure,' semiotics is not even(More)
  • 1