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Efficient L-lactic acid production from Jerusalem artichoke tubers by Lactobacillus casei G-02 using simultaneous saccharification and fermentation (SSF) in fed-batch culture is demonstrated. The kinetic analysis in the SSF signified that the inulinase activity was subjected to product inhibition, while the fermentation activity of G-02 was subjected to(More)
Corynebacterium glutamicum ATCC13032 and Brevibacterium flavum JV16 were engineered for l-valine production by over-expressing ilvEBN r C genes at 31 °C in 72 h fermentation. Different strategies were carried out to reduce the by-products’ accumulation in l-valine fermentation and also to increase the availability of precursor for l-valine biosynthesis. The(More)
Dried ground leaves of Psidium guajava L. (guava) were extracted by water and aqueous ethyl alcohol 50% (1:10) ratio, and the total phenolic content in the extracts was determined spectrophotometrically according to Folin- Ciocalteu's phenol method and calculated as gallic acid equivalent (GAE). Remarkably high total phenolic content 575.3 +/-15.5 and(More)
Zearalenone (ZEN) contamination of corn and cereal products is a serious health hazard throughout the world and its elimination by microbial methods is now being widely examined. In this study, an Aspergillus niger strain, FS10, isolated from Chinese fermented soybean, was shown to reduce levels of ZEN in corn steep liquor (CSL). Spores, mycelium and(More)
A monolithic double-balanced graphene mixer integrated circuit (IC) has been successfully designed and fabricated. The IC adopted the cross-coupled resistive mixer topology, integrating four 500 nm-gate-length graphene field-effect transistors (GFETs), four on-chip inductors, and four on-chip capacitors. Passive-first-active-last fabrication flow was(More)
Brevibacterium flavum ATCC14067 was engineered for l-valine production by overexpression of different ilv genes; the ilvEBNrC genes from B. flavum NV128 provided the best candidate for l-valine production. In traditional fermentation, l-valine production reached 30.08 ± 0.92 g/L at 31°C in 72 h with a low conversion efficiency of 0.129 g/g. To further(More)
Aspergillus niger SL-09 and Lactobacillus sp. G-02 were used as a mixed culture in a 7-l fermentor to directly form L-lactic acid from Jerusalem artichoke tubers. The synthesis of inulinase and invertase from A. niger SL-09 was enhanced significantly by the inoculation of Lactobacillus sp. G-02 at 12h of culture, which reached 275.6 and 571.8 U/ml in 60 h,(More)
The neuroprotective effects of torularhodin against oxidative injury and apoptosis in PC12 cells, as well as the related mechanisms, were investigated. The results showed that torularhodin significantly reduced lactate dehydrogenase (LDH) release and malondialdehyde (MDA) production, meanwhile increased the activities of antioxidant enzymes, which were(More)
Torulene and torularhodin are two of the principal carotenoids in Sporidiobolus pararoseus and have a similar structure to that of lycopene. The present study was to elucidate the anti-cancer activity of torulene and torularhodin in vivo with lycopene as a control. Nude mice were orally supplemented every day with a low or high dose [9 or 18 mg/kg body(More)
A method for the simultaneous replacement of a given gene by a target gene, leaving no genetic markers, has been developed. The method is based on insertional inactivation and double-crossover homologous recombination. With this method, the lysC(T311I), fbp and ddh genes were inserted into Corynebacterium glutamicum genome, and the pck, alaT and avtA genes(More)