Haydee Viloria de Castejón

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Confocal laser scanning microscopy of hamster cerebellar granular layer showed in montages of z-series the presence of small, medium and large granule cells. A granule cell Golgi cell ratio of 50/4 was observed surrounding glomerular regions. Field emission high resolution scanning electron microscopy of mouse cerebellar granular and molecular layers showed(More)
Samples of albino mice were processed by the cryofracture method for scanning electron microscopy and examined with the field emission scanning electron microscope (FESEM). Freeze-etching direct replicas of mice cerebellar cortex were also studied with the transmission electron microscope (FFTEM), as a complementary technique for obtaining higher(More)
PRIMARY OBJECTIVE The anaerobic mobilization of astrocyte glycogen in anoxic-ischemic regions of the oedematous human cerebral cortex is analysed. METHODS AND PROCEDURES Seventeen cortical biopsies of patients with brain trauma, brain tumours and congenital malformations were examined by conventional transmission electron microscopy. RESULTS(More)
The intracortical pathways of climbing fibers were traced in several vertebrate cerebella using light microscopy, confocal laser scanning microscopy, scanning and transmission electron microscopy. They were identified as fine fibers up to 1(micron thick, with a characteristic crossing-over bifurcation pattern. Climbing fiber collaterals were(More)
A correlative microscopic study of vertebrate cerebellar mossy fiber glomeruli has been carried out to obtain a three-dimensional view of the multisynaptic contacts formed by afferent mossy fibers with the granule and Golgi cell dendrites and by the monosynaptic relationship of Golgi cell axonal ramifications with granule cell dendrites. Samples of mice,(More)
The present review summarizes the outer and inner surface features of mossy fiber glomeruli in vertebrate cerebellar granular layer as seen by conventional scanning electron microscopy (SEM) and SEM freeze-fracture method. The intracortical trajectory of mossy fibers and their synaptic contacts with granule cell dendrites were traced by the slicing and(More)
Mouse and teleost fish cerebelli were processed by the freeze-fracture methods for scanning and transmission electron microscopy in order to study the three-dimensional morphology and intramembrane features of climbing fiber-Purkinje spine synapses. In addition, Alcian Blue and ruthenium chloride stainings were applied to mouse cerebellar tissue to(More)
Double fluorescent labelling of rat cerebellar cortex using antibody to glial fibrillary acidic protein (GFAP) and Alexa fluor conjugates for secondary detection for confocal laser scanning microscope (CLSM), field emission scanning electron microscopy (FESEM) of Rhesus monkey cerebellar cortex, ultrathin sectioning and freeze-etching replica method for(More)
PRIMARY OBJECTIVE Brain cortical biopsies of two patients with clinical diagnosis of complicated brain trauma who had seizures, were studied by means of light and electron microscopes in order to correlate structural alterations with seizure activity. METHODS AND PROCEDURES Biopsy samples of left frontal cortex and right parietal cortex were processed by(More)
The cerebellar basket cells of mice, hamsters, teleost fishes and human have been studied by means of Golgi light microscopy, confocal laser scanning microscopy, scanning and transmission electron microscopy. Golgi light microscopy showed ascending dendrites toward the molecular layer and descending and transverse axonal collaterals contributing to the(More)