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INTRODUCTION Embryo biopsy has potential applications in molecular research processes in domestic animals, besides its application in sex determination in embryo transfer programs. The objective of the present study was to assess the in vitro development of bovine embryos biopsied on different days of precompacted morula stage. MATERIALS AND METHODS(More)
The aim of this study was to compare the effect of time of parthenogenetic activation (22 hr versus 27 hr after In Vitro Maturation-IVM) on in vitro development of ovine oocytes using either single (Ionomycin 5 µM for 5 min or Ethanol 7% for 7 min) or combined (ionomycin and ethanol with 6-DMAP 2 mM for 3 hr) activation treatments. The abattoir-derived in(More)
BACKGROUND Oocyte maturation and subsequent in vitro production (IVP) of embryos are affected by diverse groups of chemicals in maturation medium which are needed for successful mammalian oocyte maturation during which the dramatic cytoplasmic and nuclear reprogramming events take place. This study was designed to evaluate the effects of protein source(More)
Sertoli cells are an essential group of cells in seminiferous epithelium which provide nutritional and structural supports for spermatogenic cells via cell junctions. In this study, the gene expression of connexin-43, the most abundantly distributed gap junction protein of cells, was investigated in ram Sertoli cells under mild and severe heat stresses with(More)
Maturing degree-days, gonadal histology, and changes in serum sex steroids (progesterone, P; testosterone, T; and 17β-estradiol, E2) were examined in maturing and mature spring migrant Caspian lamprey Caspiomyzon wagneri (Kessler, 1870) in the Shirud River (southern Caspian Sea). Blood and gonad samples were collected from ten fish when they first entered(More)
Divorce is an effect of several complicated psychosocial causes. It is an obvious reason for the underlying conflicts, lacking the balance and harmony of a relationship, which lead couples through a decision making process to end up their marriage. In all cultures, divorce has not been welcome. Reviewing statistics and studies on its causes that strived to(More)
To create the final spatial information and analysis, flood hazard maps and land development priority maps and information, data for the flood events to 2009 in north of Iran were incorporated with using Geo-spatial Information System data of physiographic divisions, geologic divisions, land cover classification, elevation, drainage network, administrative(More)
Improvements on embryo micromanipulation techniques led to the use of embryo biopsy in commercial embryo transfer programs for genetic analysis of preimplantation bovine embryos. The aim of this study was to evaluate the quality of bovine blastocyst derived from embryos biopsied at different pre-compacted morulae stages by assessment of cryosurvivability of(More)
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