Hasmukh V Patel

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Lateral assemblies of sphingolipids, glycosphingolipids and cholesterol, termed rafts, are postulated to be present in biological membranes and to function in important cellular phenomena. We probed whether rafts are heterogeneous by determining the relative distribution of two gangliosides, GM1 and GD3, in artificial supported monolayers, in intact rat(More)
Cardiotoxins (CTXs) from cobra venom show cytotoxicity toward several cell types. They cause systolic heart arrest and severe tissue necrosis. Their interaction with phospholipids is established but by itself fails to explain the specificity of these toxins; other component(s) of membrane must, therefore, intervene to direct them toward their target. We(More)
Thermogenin is a mitochondrial inner membrane protein which mediates non-shivering thermogenesis in BAT. It has a molecular mass of 32,000 Da and acts by uncoupling mitochondria to produce heat. We report a method of measuring its synthesis. Polysomes were isolated from BAT of developing rabbits and translated in a reticulocyte protein synthesizing system.(More)
Bruton's tyrosine kinase (BTK) plays an important role in B cell development. Deletion of C-terminal 14 amino acids of the SH3 domain of BTK results in X-linked agammaglobulinemia (XLA), an inherited disease. We report here on the stability and folding of SH3 domain of BTK. Peptides corresponding to residues 216-273 (58 residues) and 216-259 (44 residues)(More)
X-linked agammaglobulinemia (XLA), an inherited disease, is caused by mutations in the Bruton's tyrosine kinase (BTK). The absence of functional BTK leads to failure of B-cell differentiation; this incapacitates antibody production in XLA patients, who suffer from recurrent, sometimes lethal, bacterial infections. BTK plays an important role in B-cell(More)
Heparin and heparan sulfate have recently been shown to bind to snake cardiotoxin (CTX) and to potentiate its penetration into phospholipid monolayer under physiological ionic conditions. Herein we analyze the heparin-binding domain of CTX using 10 CTXs from Taiwan and African cobra venom. We also performed computer modeling to obtain more information of(More)
We have reported that some cardiotoxins (CTXs), homologous basic polypeptides of cobra venom, bind strongly to heparin. Herein we show that CTXs from spitting cobra venom bind avidly to chondroitin-6-sulfate (CS6) and dermatan sulfate (DS), the glycosaminoglycans (GAGs) abounding in the cornea and elsewhere. We compared the binding strength of Tgamma, a(More)
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