Harvey D. Preisler

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DMSO and other cryoprotective agents produce a pronounced increase on the phase transition temperature of phospholipid membranes, indicating an increased stability. The effects of DMSO and other cryoprotective agents, divalent cations, and local anaesthetics on the transition temperature of phospholipid membranes seem to correlate with their effects on the(More)
Cell cycle characteristics including labeling indices (LI), duration of S-phase (Ts), and total cell cycle time (Tc) were determined in 54 standard-risk, newly diagnosed patients with acute myeloid leukemia following an infusion of bromodeoxyuridine. Remission induction therapy consisting of cytosine arabinoside and daunomycin was then administered to all(More)
Leukemic cells were obtained from the bone marrow or peripheral blood of patients with acute myelocytic leukemia or one of its variants. The tritiated thymidine labeling index of the leukemic cells and the suicide index of the cells that produce clusters in vitro were compared. The suicide index was 5 times greater than the labeling index (S.E.) [54 +/- 8%(More)
Histone H3 mRNA in situ hybridization was compared to a reference method, iododeoxyuridine (IdUrd) immunohistochemistry of tissues labeled in vivo, as a means for assessing the proportion of S-phase cells (labeling index, LI) in oral tumor and normal mucosa. Paraffin sections from 16 patients with oral squamous cell carcinoma were studied. Patients received(More)
A comparison was made between the agar and methylcellulose culture systems with respect to their ability to support the clonal growth of leukemic cells obtained from patients with acute myeloblastic leukemia, acute lymphoblastic leukemia, and chronic myelogenous leukemia in blastic crisis. The number of clusters and/or colonies formed and the morphology of(More)
Patients with acute nonlymphocytic leukemia were given remission induction therapy consisting of cytosine arabinoside and an anthracycline. Those patients who experienced complete remission received two courses of consolidation therapy and were randomized to receive maintenance therapy consisting of either daily chemotherapy with reinforcements every 3 mo(More)
Advances in the treatment of acute myelocytic leukemia (AML) have largely resulted from empirical drug trials in man. Such trials have led to the recognition of drug combinations which pro du ce remissions in more than one-half of the patients who are under 70 years of age (Yates et al. 1973; Preisler et al. 1977; Rees et al. 1977; Jacquillat et al. 1976).(More)
Bone marrow cells obtained from 166 patients with acute nonlymphocytic leukemia were cloned in vitro. The number and size of clones produced differed among patients and was unrelated to French-American-British type of leukemia, to patient age, to whether the patient was studied at the time of initial diagnosis or at relapse, or to the cytogenetic (normal or(More)
The vast majority of murine leukaemias are lymphoid in nature. They have been extensively used in chemotherapy studies, and the data obtained have to some extent been applicable to the treatment of mye-loid leukaemia in man, but their lymphoid nature has prevented them from being used to study the relationship between myeloid leukaemic cells and normal(More)