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Two cell dispersion methods for excised goldfish pituitary glands were tested, and a cultured dispersed cell system based on trypsin enzymatic tissue digestion was developed and characterized. Controlled trypsin/DNase treatment of goldfish pituitary gland yielded dispersed cells of high viability (trypsin blue exclusion test) that responded to gonadotropin(More)
Dispersed pituitary cells of the goldfish were incubated with biotinylated [D-Lys6, Pro9-N-ethylamide] salmon gonadotropin-releasing hormone (sGnRH-A) then avidingold (10 nm), and were fixed, embedded and sectioned. Cells were identified as gonadotrophs, somatotrophs, or prolactin cells using specific hormone antisera and protein-A gold (20 nm) as a marker.(More)
The immunocytological activity of an antiserum to carp growth hormone was studied on the goldfish pituitary gland, using a peroxidase-antiperoxidase ultrastructural technique. The reaction was specific for secretory granules in cells in the proximal pars distalis categorized as growth hormone cells on the basis of ultrastructural characteristics. The(More)
We describe, for the first time in insects, the presence of serotonin-like immunoreactive dorsal unpaired median (DUM) neurons. In unfed, untreated Rhodnius prolixus, the cell bodies of these DUM neurons displayed only faint serotonin-like immunofluorescence without any detectable fluorescence in the axons. There was, however, an extensive serotonin-like(More)
The fine structure of the various hormone-producing cell types (with the exclusion of the prolactin cells) in the pituitary gland (pars distalis) of migratory sockeye salmon is described. All fish were in an advanced stage of sexual maturation. In the proximal pars distalis five cell types were distinguished: growth hormone cells, ACTH cells, gonadotrops,(More)
The effect of exogenous testosterone on the synthesis and release of a bioactive gonadotropic hormone-releasing hormone (GnRH)-like substance has been studied in juvenile male rainbow trout. Long-term testosterone treatment caused an increased gonadotropic hormone (GTH) release and precocious sexual maturity. Tel- and diencephalon extracts from long-term(More)
Dorsal roots are absent from the tails of Xenopus larvae. Sensory afferents instead enter the spinal cord via the ventral roots. After reaching the cord these axons travel diagonally within the lateral fasciculus of the cord to reach the dorsolateral fiber bundle in which they ascend to the hindbrain. Sensory afferents entering together in the same ventral(More)