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  • Haruhiko Sakuraba, Issei Yoshioka, +5 authors Toshihisa Ohshima
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 2002 (First Publication: 12 April 2002)
  • A gene encoding an ADP-dependent phosphofructokinase homologue has been identified in the hyperthermophilic archaeon Methanococcus jannaschii via genome sequencing. The gene encoded a protein of 462Expand
  • Haruhiko Sakuraba, Kazunari Yoneda, +7 authors Toshihisa Ohshima
  • Medicine, Chemistry
  • Applied and Environmental Microbiology
  • 2007 (First Publication: 28 September 2007)
  • ABSTRACT Genes encoding 2-deoxy-d-ribose-5-phosphate aldolase (DERA) homologues from two hyperthermophiles, the archaeon Pyrobaculum aerophilum and the bacterium Thermotoga maritima, were expressedExpand
  • Takenori Satomura, Ryushi Kawakami, Haruhiko Sakuraba, Toshihisa Ohshima
  • Biology, Medicine
  • The Journal of Biological Chemistry
  • 2002 (First Publication: 12 April 2002)
  • The activity of dye-linkedd-proline dehydrogenase was found in the crude extract of a hyperthermophilic archaeon, Pyrobaculum islandicum JCM 9189. The dye-linked d-proline dehydrogenase was aExpand
  • Hideaki Tsuge, Ryushi Kawakami, +5 authors Toshihisa Ohshima
  • Biology, Medicine
  • Journal of Biological Chemistry
  • 2005 (First Publication: 2 September 2005)
  • Two novel types of dye-linked l-proline dehydrogenase complex (PDH1 and PDH2) were found in a hyperthermophilic archaeon, Pyrococcus horikoshii OT3. Here we report the first crystal structure ofExpand
  • Haruhiko Sakuraba, Ryushi Kawakami, Toshihisa Ohshima
  • Medicine, Biology
  • Applied and Environmental Microbiology
  • 2005 (First Publication: 1 August 2005)
  • ABSTRACT The gene (PH1074) encoding the NAD kinase of the hyperthermophilic archaeon Pyrococcus horikoshii was identified in the genome database, cloned, and functionally expressed in EscherichiaExpand
  • Haruhiko Sakuraba, Tomoyuki Kawai, Kazunari Yoneda, Toshihisa Ohshima
  • Biology, Medicine
  • Archives of biochemistry and biophysics
  • 2011 (First Publication: 15 August 2011)
  • The crystal structure of a highly thermostable UDP-galactose 4-epimerase (GalE) from the hyperthermophilic archaeon Pyrobaculum calidifontis was determined at a resolution of 1.8Å. The asymmetricExpand