Harminder S Dua

Learn More
The amniotic membrane is the innermost of the three layers forming the fetal membranes. It was first used in 1910 in skin transplantation. Thereafter it has been used in surgical procedures related to the genito-urinary tract, skin, brain, and head and neck, among others. The first documented ophthalmological application was in the 1940s when it was used in(More)
Stem cells have certain unique characteristics, which include longevity, high capacity of self-renewal with a long cell cycle time and a short S-phase duration, increased potential for error-free proliferation, and poor differentiation. The ocular surface is made up of two distinct types of epithelial cells, constituting the conjunctival and the corneal(More)
BACKGROUND/AIMS There is substantial evidence that mammalian epithelial stem cells are located within well defined niches. Although the corneoscleral limbus is acknowledged as the site of corneal epithelial stem cells no anatomical niche for such cells has yet been described. The authors undertook to re-evaluate the microanatomy of the limbus in order to(More)
PURPOSE To develop current treatment recommendations for dry eye disease from consensus of expert advice. METHODS Of 25 preselected international specialists on dry eye, 17 agreed to participate in a modified, 2-round Delphi panel approach. Based on available literature and standards of care, a survey was presented to each panelist. A two-thirds majority(More)
PURPOSE To define and characterize a novel pre-Descemet's layer in the human cornea. DESIGN Clinical and experimental study. PARTICIPANTS We included 31 human donor sclerocorneal discs, including 6 controls (mean age, 77.7 years). METHODS Air was injected into the stroma of donor whole globes (n = 4) and sclerocorneal discs (n = 21) as in the clinical(More)
INTRODUCTION Amniotic membrane (AM) has gained increasing popularity as a useful carrier for ex vivo-expanded cells for tissue engineering, particularly in ocular surface reconstruction. However, current methods employed for denuding AM are highly variable, and the consequent effects on the structural and molecular composition of the AM basement membrane(More)
PURPOSE To compare intraocular pressure (IOP) measurements of the Ocular Blood Flow (OBF) pneumotonometer, Goldmann applanation tonometer, and Tono-Pen in eyes with normal corneas of various thicknesses. METHODS IOP was measured with an OBF pneumotonometer, Tono-Pen and Goldmann applanation tonometer in random order in 181 eyes with normal corneas.(More)
PURPOSE To evaluate dendritic cell (DCs) density, distribution, and morphology in central corneal and limbal epithelium in normal subjects and patients with immune-mediated corneal inflammation using in vivo confocal microscopy (IVCM). DESIGN Comparative case-controlled, observational confocal microscopy study. METHODS A total of 135 eyes of 135(More)
CD34 is a transmembrane phosphoglycoprotein, first identified on hematopoietic stem and progenitor cells. Clinically, it is associated with the selection and enrichment of hematopoietic stem cells for bone marrow transplants. Due to these historical and clinical associations, CD34 expression is almost ubiquitously related to hematopoietic cells, and it is a(More)
PURPOSE Sodium hyaluronate (hyaluronic acid) is known to promote corneal epithelial wound healing in vivo and in vitro, in animal experiments. Sodium hyaluronate is the ligand for CD44, a cell surface adhesion molecule which has been found on normal human corneal epithelial cells. The purpose of this study was to investigate the effect of sodium hyaluronate(More)