Harm-Anton Klok

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This feature article provides a compilation of tools available for preparing well-defined peptide/protein-polymer conjugates, which are defined as hybrid constructs combining (i) a defined number of peptide/protein segments with uniform chain lengths and defined monomer sequences (primary structure) with (ii) a defined number of synthetic polymer chains.(More)
In the past decades, polymeric nanoparticles have emerged as a most promising and viable technology platform for targeted and controlled drug delivery. As vehicles, ideal nanoparticles are obliged to possess high drug loading levels, deliver drug to the specific pathological site and/or target cells without drug leakage on the way, while rapidly unload drug(More)
2.5.6. (Selective) Chain End Postmodification 5494 3. Characterization 5496 4. Properties and Applications of Polymer Brushes 5498 4.1. Responsive Surfaces 5498 4.1.1. Solvent Responsive Polymer Brushes 5498 4.1.2. Thermoresponsive Polymer Brushes 5500 4.1.3. pHand Ion-Sensitive Polymer Brushes 5502 4.2. Nonbiofouling Surfaces 5504 4.2.1. Neutral(More)
This report demonstrates the feasibility of surface-initiated atom transfer radical polymerization to prepare thin polymer layers ("brushes") that can be functionalized with short peptide ligands and which may be of use as coatings to promote endothelialization of blood-contacting biomaterials. The brushes are composed of poly(2-hydroxyethyl methacrylate)(More)
The feasibility of a new transfection agent, HBPL, for the production of recombinant IgG antibody via TGE as well as for the transfection of primary cells is studied. Under the conditions investigated, transfection of CHO-DG44 cells using HBPL results in IgG yields that are comparable to those obtained with PEI. In experiments with CHO-K1 cells and MEFs the(More)
The conjugation of peptides/proteins and synthetic polymers is a useful strategy to overcome some of the limitations related to the use of the individual components. This review will highlight two aspects: enhanced structural control at the nanometer level and improved performance, in particular with respect to biomedical applications. In the former case,(More)
A new strategy for the preparation of protein-functionalized polymer brushes is reported, which is based on a combination of surface-initiated atom transfer radical polymerization (ATRP), p-nitrophenyl chloroformate activation of the surface hydroxyl groups, and subsequent O(6)-benzylguanine (BG) functionalization. The BG-functionalized brushes are used to(More)
This manuscript reports a facile strategy for the modification of polyethylene, a widely used biomaterial, with a thin non-biofouling coating that can act as a platform to introduce biochemical cues to control and direct, e.g., cell adhesion and proliferation. The non-biofouling coating is produced, following a two-step strategy involving photobromination(More)
This contribution investigates the effects of chain length and chemical composition on the secondary structure and LCST behavior of a library of short, elastin-like peptides based on the GVGVP motif. CD experiments revealed that most of the investigated peptides showed the typical elastin conformational behavior with a decrease in random coil and an(More)