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The extract of the plant Rheum officinale Baill, mainly containing the anthraquinones physcion and chrysophanol, is highly active against plant powdery mildew. Experiments were conducted in the laboratory and greenhouse to determine the interaction of the two compounds on cucumber powdery mildew [Sphaerotheca fuliginea (Schlecht.) Poll] and on wheat powdery(More)
The reliable gene expression analysis of a reverse transcription quantitative polymerase chain reaction (qRT-PCR) mainly depends on selecting suitable reference genes that can express stably under different experimental conditions. Thus far, no reference genes have been identified in flixweed. In this paper, 7 supposed reference genes were selected to(More)
Department of Plant Pathology, College of Plant Protection, China Agricultural University, Beijing, 100193, China; State Key Laboratory of Plant Physiology and Biochemistry, College of Biological Sciences, China Agricultural University, Beijing 100193, China; National Engineering Research Center for Vegetables, Beijing Academy of Agriculture and Forestry(More)
The acetolactate synthase (ALS) inhibitor tribenuron has been used continuously for approximately twenty years as an herbicide in winter wheat fields in China. Flixweed (Descurainia sophia) has evolved resistance to tribenuron, due to multiple amino acid mutations at the 197th residue of ALS. In this study, the molecular basis of tribenuron resistance was(More)
BACKGROUND Physcion is a key active ingredient of the ethanol extract from roots of Chinese rhubarb (Rheum officinale Baill.) that has been commercialised in China for controlling powdery mildews. The biological mechanism of action of physcion against the barley powdery mildew pathogen was studied using bioassay and microarray methods. RESULTS Bioassay(More)
Isothiocyanates (ITCs) are one of the many classes of breakdown products of glucosinolates found in plants and exhibit biologic activity against various pathogens. In this work, aliphatic isothiocyanates were prepared and the antimicrobial activities against plant pathogenic fungi and bacteria were tested to understand the structure–activity relationships.(More)
This paper studied the impact of several herbicides on the conidium germination of Exserohilum monoceras strain X27 in petri dish, and the synergistic effects of the pathogen and chemical herbicide quinclorac or propanil against Echinochloa crus-galli in greenhouse. The prevention efficiency of the tank-mixture of pathogen and quinclorac was also evaluated(More)
BACKGROUND Glyphosate is a non-selective, foliar-applied, systemic herbicide that kills weeds by inhibiting the synthesis of 5-enolpyruvylshikimate-3-phosphate synthase. Urea phosphate (UPP), made by the reaction of urea with phosphoric acid, was applied as an adjuvant for glyphosate in this study. Experiments in the greenhouse and the field were conducted(More)
Exserohilium monoseras is a potential agent for barnyardgrass (Echinochlao crus-galli) control. This study was to evaluate the genetic diversity of this fungus with 17 strains isolated from different rice growing regions of China by the random amplified polymorphic DNA (RAPD). Among 25 arbitrary primers, 20 primers could get enough amplified bands for all(More)
Physcion and chrysophanol induce defense responses against powdery mildew in cucumbers. The combination of these two compounds has synergistic interaction against the disease. We performed RNA-seq on cucumber leaf samples treated with physcion and chrysophanol alone and with their combination. We generated 17.6 Gb of high-quality sequencing data (∼2 Gb per(More)