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Seasonal time of switchgrass (Panicum virgatum L.) harvest affects yield and biofuel quality and balancing these two components may vary depending on conversion system. A field study compared fall and spring harvest measuring biomass yield, element concentration, carbohydrate characterization, and total synthetic gas production as indicators of biofuel(More)
Improving digestibility of roughage cell walls will improve ruminant animal performance and reduce loss of nutrients to the environment. The main digestibility impediment for dicotyledonous plants is highly lignified secondary cell walls, notably in stem secondary xylem, which become almost non-digestible. Digestibility of grasses is slowed severely by(More)
BACKGROUND Alfalfa, [Medicago sativa (L.) sativa], a widely-grown perennial forage has potential for development as a cellulosic ethanol feedstock. However, the genomics of alfalfa, a non-model species, is still in its infancy. The recent advent of RNA-Seq, a massively parallel sequencing method for transcriptome analysis, provides an opportunity to expand(More)
NMR methods can be used to delineate detailed structural and regiochemical information on the plant cell wall and elucidate biochemical incorporation pathways. Maize lignin isolated in high yield from rind tissue of stem internodes contained high amounts of esterified p-coumaric acid. Available literature indicated that acylation of the lignin by p-coumaric(More)
BACKGROUND The GeneChip(R) Medicago Genome Array, developed for Medicago truncatula, is a suitable platform for transcript profiling in tetraploid alfalfa [Medicago sativa (L.) subsp. sativa]. However, previous research involving cross-species hybridization (CSH) has shown that sequence variation between two species can bias transcript profiling by(More)
Tilman et al. (Reports, 8 December 2006, p. 1598) argued that low-input high-diversity grasslands can provide a substantial proportion of global energy needs. We contend that their conclusions are not substantiated by their experimental protocol. The authors understated the management inputs required to establish prairies, extrapolated globally from(More)
Evaluation of biomass crops for breeding or pricing purposes requires an assay that predicts performance in the bioenergy conversion process. Cell wall polysaccharide hydrolysis was compared for a dilute sulfuric acid pretreatment at 121°C followed with cellulase hydrolysis for 72 h conversion assay (CONV) with in vitro rumen microflora incubation for 72 h(More)
Maize (Zea mays L.) stover and cobs are potential feedstock sources for cellulosic ethanol production. Nitrogen (N) fertilization is an important management decision that influences cellulosic biomass and grain production, but its effect on cell wall composition and subsequent cellulosic ethanol production is not known. The objectives of this study were to(More)
Alfalfa hay is a major crop supporting U.S ruminant livestock industry, particularly dairy. Several cellulosic feedstocks will be needed to meet current ethanol production goals. Alfalfa has considerable potential as a feedstock for production of ethanol and other industrial materials because of its high biomass production, perennial nature, ability to(More)