Hans Hultberg

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Thirty-two Escherichia coli strains from 30 children with pyelonephritis were examined for their haemagglutination patterns and O and K serotypes. 29 (91%) of the strains showed mannose-resistant haemagglutination (MRHA). By use of well-defined target cells, these MRHA+ strains could be shown to recognise human cells either in a P-specific manner(More)
Several fusions between the gene for human insulin-like growth factor I (IGF-I) and the genes for different IgG-binding fragments of staphylococcal protein A were assembled and compared regarding expression, secretion, and purification of the peptide hormone. After IgG affinity purification of the fusion proteins from the growth medium of Staphylococcus(More)
Earlier investigations have shown that pyelonephritic Escherichia coli specifically recognize and bind to carbohydrate structures correlated to the P blood group antigens. These findings are confirmed and extended in this study. Twenty-two of 23 nonselected E. coli strains from children with acute febrile pyelonephritis failed to agglutinate human(More)
The occurrence of Escherichia coli possessing P blood-group-specific adhesins (P-fimbriae) was examined in 97 children with urinary tract infections and 82 healthy controls. P-fimbriae were present in 91% (33/35) of the urinary strains causing acute pyelonephritis. Among strains causing cystitis and asymptomatic bacteriuria P-fimbriae were found in 19% and(More)
Two novel adenovirus-2 early region 1A mRNAs, designated 10S and 11S, have been characterized. They differ from the previously described 9S, 12S and 13S mRNAs by having an additional intron removed during mRNA maturation. The 10S and 11S mRNAs encode proteins with mol. wts of 30 and 35 kd. These proteins are encoded in the same translational reading frame(More)
Recovery from anthropogenic acidification in streams and lakes is well documented across the northern hemisphere. In this study, we use 1996-2009 data from the four Swedish Integrated Monitoring catchments to evaluate how the declining sulfur deposition has affected sulfate, pH, acid neutralizing capacity, ionic strength, aluminum, and dissolved organic(More)
The binding of pyelonephritogenic Escherichia coli strains to human uroepithelial cells from patients with and without P blood group antigens was investigated. Uroepithelial cells from p phenotypes bound pyelonephritogenic e. coli to a significantly lesser extent than did cells from P1 and P2 phenotypes. The binding of pyelonephritogenic E. coli to urinary(More)
P-antigen-recognizing fimbriae (P fimbriae) from four pyelonephritogenic Escherichia coli strains and type 1 fimbriae from an E. coli strain and a Salmonella typhimurium strain were purified. The P fimbriae were morphologically similar to type 1 fimbriae. The purified P fimbriae agglutinated neuraminidase-treated human P1 and P2k erythrocytes but not p(More)
Soil water chemistry in forest soils over 20 years was studied at nine sites in southern Sweden. The aim was to investigate the recovery from acidification and the influence of strong sea salt episodes that occur in the region. All sites but one showed signs of recovery from acidification along with the reduced sulphur deposition, but the recovery progress(More)
Most (greater than 90%) Escherichia coli strains isolated from children with acute non-obstructive pyelonephritis exhibit a specific type of filamentous protein appendage known as P-fimbriae. These fimbriae enable the bacterium to adhere to human uroepithelial cells by the specific recognition of and binding to a particular class of glycosphingolipids(More)