Hans - Guido Wendel

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We developed mice with germline endogenous expression of oncogenic Hras to study effects on development and mechanisms of tumor initiation. They had high perinatal mortality, abnormal cranial dimensions, defective dental ameloblasts, and nasal septal deviation, consistent with some of the features of human Costello syndrome. These mice developed papillomas(More)
Earlier studies from this laboratory have shown that the uricosuric agent probenecid (PBCD) will inhibit the extrusion of folate analogues from tumor cells mediated by a plasma membrane ATPase resembling the canicular multispecific organic anion transporter/multidrug resistance-related protein (MRP) family of ATP binding cassette transporters. This(More)
Deregulation of protein translation is a common event in cancer and occurs frequently as a result of mutational activation of the AKT signaling pathway. We had previously reported the in vivo oncogenic activity of the translation initiation factor eIF4E, which acts downstream AKT and mTOR. We now identified an absolute requirement for Ser209 phosphorylation(More)
Transfected cells were washed with PBS and treated with RIPA buffer with no SDS. Mouse monoclonal antibody against Flag (M2, Sigma) was added to cell extract and incubated overnight at 4 8C. Protein-A-conjugated Sepharose equilibrated in RIPA was then added and incubated for 2 h. The beads were extensively washed with ice-cold RIPA and the precipitate was(More)
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