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The kinetics of glucose transport and the transcription of all 20 members of the HXT hexose transporter gene family were studied in relation to the steady state in situ carbon metabolism of Saccharomyces cerevisiae CEN.PK113-7D grown in chemostat cultures. Cells were cultivated at a dilution rate of 0.10 h-1 under various nutrient-limited conditions(More)
To increase the power and the robustness of spectroscopic process analyzers, methods are needed that suppress the spectral variation that is not related to the property of interest in the process stream. An approach for the selection of a suitable method is presented. The approach uses the net analyte signal (NAS) to analyze the situation and to select(More)
The qualitative and quantitative aspects of capillary electrophoretic methods used to study drug-protein interactions, viz. the affinity capillary electrophoresis (ACE). Hummel-Dreyer (HD), frontal analysis (FA), vacancy peak (VP) and vacancy affinity capillary electrophoresis (VACE) methods have been investigated. In the ACE and the VACE methods the(More)
MOTIVATION Metabolomics datasets are generally large and complex. Using principal component analysis (PCA), a simplified view of the variation in the data is obtained. The PCA model can be interpreted and the processes underlying the variation in the data can be analysed. In metabolomics, often a priori information is present about the data. Various forms(More)
A new method to eliminate the background spectrum (EBS) during analyte elution in column liquid chromatography (LC) coupled to spectroscopic techniques is proposed. This method takes into account the shape and also intensity differences of the background eluent spectrum. This allows the EBS method to make a better estimation of the background eluent(More)
Determination of homogeneous mixing of the active pharmaceutical ingredient (API) is an important in-process control within the manufacturing of solid dosage forms. In this paper two new near-infrared (NIR) based methods were presented; a qualitative and a quantitative method. Both methods are based on the calculation of net analyte signal (NAS) models(More)
Fast and accurate monitoring of monomer concentration during copolymerization reactions is of much interest. It is known that near-infrared spectroscopy (NIRS) can be used to monitor polymerization reactions. Here, a free radical solution copolymerization reaction between methyl methacrylate and N,N-dimethylacrylamide is considered. NIR spectra were(More)
The applicability of capillary zone electrophoresis (CZE) for the determination of association constants of an hapten-antibody complex with values as high as 10(7) (mol/I)-1 was investigated (dissociation constant in the nmol range). As a reference method the well known, enzyme-linked immunosorbent assay (ELISA) was selected. The study describes the(More)
The 'gap' between the discovery and the optimisation phases in high-throughput systems is described and the problems that stem from the centralised nature of parallel reactor setups are outlined. It is proposed that on-line analysis methods (e.g. fast spectroscopic analysis) can be adapted to perform kinetic studies in high-throughput environments.(More)
The importance of small metal clusters in catalysis and the problems in understanding the clustering process in solution are outlined. A new analysis method for UV/Vis spectra is presented and applied to monitor the kinetics of ion reduction and cluster formation in situ. This method, which is based on a combination of two chemometric techniques, takes into(More)