Hans A. Biebuyck

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Self-assembled monolayers (SAMs) form when organic molecules spontaneously chemisorb on the surfaces of solids (e.g. organic thiols and disulfides on gold, silver, and copper or carboxylic acids on the surface of alumina).1'2 The most robust and best characterized SAMs are those comprising alkanethiolates on gold.l By variation of the length of the alkane(More)
--,9$e-r9d,^o-r-gary-c.Tgnolay_ers^we1e {o^rae_{ 9ng9ld slides by adsorption from ethanol of HS(CH)'.CH2OH, HS(CH2)1oCHg, [S(CHilIoCH2OH]2, [S(CHtroeH3]2, and binary .ni"t.r.". of these molecules in whictr one component was terminated by a hydrophobic methy_l group and one by a hydrophilic alcohol group. The compositions of the monolayers were determirqe$(More)
Microfluidic networks (microFNs) were used to pattern biomolecules with high resolution on a variety of substrates (gold, glass, or polystyrene). Elastomeric microFNs localized chemical reactions between the biomolecules and the surface, requiring only microliters of reagent to cover square millimeter-sized areas. The networks were designed to ensure(More)
This paper outl ines a general s1'nthetic route to alkanethiols useful in forming self-asseryl ied monolayers (SAMsis tar t ing f rom amines. RNH:. and conver t ing them. r ' ia cr -ch loroamides RNHCOCHzCI, to th io ls hav ing the s t ructure RNHCOCH2SH. The u 'e t tab i l i t res ,es t inra ted f ron i contact ang les o f rvater and hexadecane) and the th(More)
The blueprint for cellular diversity and response to environmental change is encoded in the cis-acting regulatory sequences of most genes. Deciphering this 'cis-regulatory code' requires multivariate data sets that examine how these regions coordinate transcription in response to diverse environmental stimuli and therapeutic treatments. We describe a(More)
This paper describes a procedure, based on competitive binding, for identifying tight-binding ligand(l)Ig a receptor in mixtures of equimolar ligand candidates using affinity capillary electrophoresis (ACE). Vancomycin and a small library of 32 peptides were used as a model syatem to illushate the procedureThis procedure should be applicable to peptide(More)
Light-coupling masks ~LCMs! based on structured organic polymers that make conformal contact with a substrate can constitute an amplitude mask for light-based lithographies. The LCM is exposed through its backside, from where the light is differentially guided by the structures towards the substrate. Images of arbitrarily shaped features having dimensions(More)
Biological experiments at the solid/liquid interface, in general, require surfaces with a thin layer of purified molecules, which often represent precious material. Here, we have devised a method to extract proteins with high selectivity from crude biological sample solutions and place them on a surface in a functional, arbitrary pattern. This method,(More)