Hanns J. Bauer

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The avian herpesviruses infectious laryngotracheitis virus (ILTV) and herpesvirus of turkeys (HVT), as well as the mammalian herpesvirus pseudorabies virus (PRV) were able to provide complete helper activity for the production of infectious avian adeno-associated virus (AAAV) in chicken cells. The presence of AAAV in the infected chicken cell reduced the(More)
Avian adeno-associated virus (AAAV) pre-purified by Uvasol extraction, one discontinuous and two CsCl equilibrium density gradients, was still considerably contaminated with avian adenovirus (CELO strain). Four different approaches were investigated in attempts to improve the elimination of the contaminating CELO virus. The contaminations were assayed by(More)
As the growth kinetics of avian adeno-associated virus (AAAV) in chicken cells demonstrate, the three serotypes of fowl adenovirus (FAV), FAV -1, -5 and -8, provide complete helper activity for the production of infectious AAAV. Under one step conditions, the growth cycle of AAAV in primary chicken kidney cell (CKC) cultures is characterised by an eclipse(More)
Using growth kinetics we demonstrate two effects based on interactions between the chicken herpesvirus, Marek's disease virus (MDV), and the dependovirus, avian adeno-associated virus (AAAV), in coinfected chicken embryo fibroblasts (CEF): (i) MDV provides helper activity for an efficient multiplication of AAAV; (ii) a high multiplicity of coinfecting AAAV(More)
Two infectious components with buoyant densities of 1.40 g/cm3 and 1.45 g/cm3, designated as major (1.40) and minor (1.45) component, were detected by banding avian adeno-associated virus (AAAV) isopycnically in CsCl. In metrizamide, however, infectious AAAV banded only as a single peak at a density of 1.32 g/cm3. Biological as well as physicochemical(More)
Using the chloroquine-modified calcium phosphate coprecipitation technique, fowl adenovirus serotype 1 (FAV 1) DNA transfects efficiently chicken cell cultures. Infection of FAV 1 DNA transfected cells with helper dependent avian adeno-associated virus (AAAV) results in the production of AAAV progeny, being detected by an indirect immunofluorescence assay.(More)
For the stress analysis of the human mandible a flexible simulation concept basing on finite element-method has been developed. One of the main issues is the prediction of fractures as a consequence of known forces as well as the forensic reconstruction of the traumatologic situation. At first, the individual geometry was reconstructed by 3D-CT-Scans. To(More)